摘要
目的 建立齐多夫啶血药浓度的反相高效液相色谱检测方法。方法 以shim pack ,VP ODS 5 μm 4 6× 15 0mm为分离柱 ,流动相∶四氢呋喃∶水∶三氟酸 (10∶89 8∶0 2 ) ,流速 1 0ml·min-1;检测波长 2 67nm。结果 齐多夫啶浓度在 0 0 2～ 2 0mg·L-1范围内与峰面积呈良好的线性关系 ,日内及日间差均小于 5 % ,平均回收率为 96 4% ,血浆中最低检测极限为 10 μg·L-1。结论 本方法具有快速、灵敏、稳定的特点 。
AIM To establish a RP HPLC method for the determination of zidovudine(AZT) plasma concentration. METHODS A shim pack,VP ODS. 5 μm 4 6×150 mm radial compression column was used with a mobile phase of tetrahydrofuran water trifluoroacetic acid(10∶89 8∶0 2). The flow rate was 1 0 ml·min -1 .The detector was operated at 267 nm. RESULTS The calibration curves of AZT were linear separately within the concentration range of 0 02～20 mg·L -1 . The relative standard deviations for within day and between days assays were all less than 5%. The relative recovery of the method were 96 4%. The limit of quantitation of the assay is 10 μg·L -1 of plasma. CONCLUSIONS The methed is rapid,accurate and stable. The determination of AZT plasma concendration could be used.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2002年第6期707-709,共3页
Chinese Pharmacological Bulletin
基金
卫生部科学研究基金资助课题 No 98 1 336
