天疱疮患者外周血单一核细胞γ干扰素、白介素10mRNA表达水平的研究

Expression of IFN-γmRNA and IL-10mRNA on Peripheral Blood Mmononuclear Cells of Patients With Pemphigus

目的探讨天疱疮患者发病期和缓解期辅助性T细胞(Th)发病模式。方法应用逆转录-聚合酶链反应(RT-PCR)检测20例天疱疮患者急性发病期、缓解期和20例正常对照外周血单一核细胞干扰素γ(IFN-γ)、白介素10(IL-10)、mRNA的表达水平。结果天疱疮患者急性发病期IFN-γmRNA水平高于正常人,差异有显著性(P<0.001);缓解期IFN-γmRNA水平与正常人差异无显著性(P=0.9800)。发病期IL-10mRNA水平低于正常人,但差异无显著性(P=0.0834)。结论天疱疮患者发病过程中有Th1/Th2细胞失平衡现象,发病期Th1型细胞因子IFN-γ可能占优势。

Objective To probe the Th cytokine pattern in pemphigus,so as to clarify the pathogenesis of pemphigus and draw up new therapy.Methods Reverse transcription-polymerase chain reaction(RT-PCR)was used to examine the interferon gamma(IFN-γ)and interleukin10(IL-10)mRNA expression on peripheral blood mononuclear cells(PBMCs)of20cases of pemphigus in acute stage,20cases in stable stage,and20normal controls.Results The IFN-γmRNA expression in20cases of pemphigus in acute stage was higher than that of controls(P<0.001),while in stable stage there was no difference with that of controls(P=0.98).IL-10mRNA expression of pemphigus in acute stage was lower than that of controls,but the difference was not significant(P=0.0834).Conclusion IFN-γmRNA expression is predominant in acute stage of pemphigus.