脾虚型胃癌腹膜移植模型的建立及其生物学特性研究

Establishment and biologic characteristic of transplantable peritoneal metastasis of human gastric cancer in nude mice with the spleen asthenia syndrome

目的 :建立一种能稳定传代的脾虚型人胃癌裸鼠腹膜移植模型 ,探讨该模型用于中西医结合抗胃癌作用研究的可行性。方法 :将裸鼠随机分为对照组、脾虚组和健脾组。首先将脾虚组和健脾组裸鼠喂饲大黄芒硝合剂 (按 4∶ 1混合 ) 5 d以建立脾虚模型 ,健脾组给予参芪扶正注射液 3d,其他组给予等量冷开水对照。然后各组分别用人胃癌 OCU M- 2 MD3可移植性细胞株腹腔移植并传代。观察肿瘤生长、腹水量、生存时间、宿主体重、进食、胃肠黏膜形态学变化 ,以及外周血自然杀伤 (NK)细胞活性、红细胞免疫功能的变化。结果 :经病理证实所有造模动物均形成腹膜广泛种植结节和癌性腹水 ,移植瘤形态学、免疫学、分子遗传学及超微结构符合肠型胃癌特点 ,经 10代观察 ,移植率为 10 0 %。与对照组相比 ,脾虚组腹膜移植瘤结节多 ,腹水生长快 ,生存期短 ,动物进食量减少 ,体重明显下降 ,胃肠黏膜出现糜烂破溃 ,NK细胞活性降低 ,免疫复合物花环率均较腹膜移植组显著增加。健脾组各项指标与脾虚组相比有明显改善。结论 :该模型可作为研究胃癌腹膜转移机制、生物学行为及中西医结合治疗的一种手段。

Objective:To establish a model for transplantable peritoneal metastasis of human gastric cancer in nude mice with the spleen asthenia syndrome.To explore the feasibility of using this model to study the anti-gastric cancer effect of combination of TCM and western medicine.Methods:The mice were divided into 3 groups,control group,the spleen asthenia syndrome group(SAS group)and the spleen tonic group(ST group).The nude mice of SAS group and ST group were fed the rhubarb and mirabilite mixture to establish the spleen asthenia syndrome models.All of the mice were implanted and passaged the gastric cancer cell OCUM-2MD3.The growth of tumor,ascites and existed time were observed.The changes of weight,quantity of food intake,morphological changes of gastrointestinal mucosa were observed.The NK cell activity and erythrocyte immune function were valued too.Results:All implanted animals developed ascites and large amount of peritoneal metastatic nodules by pathologic examination.The morphology,histochemistry and ultrastructure in xenografted tumor were in accord with the characteristic for intestinal type of gastric cancer.The transplanted rate was 100%.Compare with the control group,large amount of peritoneal metastatic nodules and ascites growth faster could be observed in SAS group.Meanwhile,mice in SAS group showed shorter existed time,decreased food intake quantity,lose of weight,decreased activity of NK cell and significant increase in RBC-ICR.Erosion and ulceration of gastrointestinal mucosa were found.All of indexs above were improved in ST group.Conclusion:This animal model was deserved further investigation as integration of disease,symptoms and signs in traditional Chinese medicine and could be used to study the mechanism,biologic behavior and treatment for peritoneal metastasis of gastric cancer combinated by TCM and western medicine.