CpG ODN增强乙型肝炎表面抗原免疫小鼠的抗体产生

CpG ODN enhance the antibody production in mice immunized with hepatitis B surface antigen

目的 :探讨合成含CpG基序的寡核苷酸 (CpGODN)对重组乙型肝炎表面抗原 (rHBsAg)及乙型肝炎疫苗增强小鼠特异性抗体产生的效应。方法 :采用非纯系 (Km)及纯系 (Balb c)小鼠作为免疫对象 ,经后腿胫骨前肌免疫 2次 ,ELISA法检测血清乙型肝炎表面抗体 (抗 HBs)效价。结果 :加CpGODN组 ,其抗 HBs效价均较单独注射rHBsAg和疫苗组明显增高 ,持续时间长 ,且纯系鼠的抗体效价明显高于非纯系鼠。结论 :CpGODN对小鼠抗 HBs产生具有增强作用 ,且与疫苗中的铝佐剂有协同效应。

Objective:To investigate the effect of synthetic oligodeoxynucleotide containing CpG motifs (CpG ODN) on antibody production to recombinant hepatitis B surface antigen (rHBsAg) or hepatitis B vaccine respectively. Methods:The antigens were injected into the back leg tibialis anterior(TA) muscle of all the mice (Km breed and Balb/c breed) for two times.The serum anti HBs titers were detected by ELISA.Results:The anti HBs titers of additional CpG ODN groups are obviously higher than those of rHBsAg group or vaccine group alone, the antibody persistent time is some longer. Nevertheless, the anti HBs titers of pure breeding mice are significantly higher than those of non pure breeding mice. Conclusion:CpG ODN has enhanced efficacy for anti HBs production in immunized mice and synergetic effect can be observed in alum adjuvant of hepatitis B vaccine.