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大鼠移植慢性排斥组织中CD40和CD40L表达研究 被引量：3

The expression of CD40/CD40L during chronic rejection of renal allograft in rats
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摘要 目的 :观察大鼠移植肾慢性排斥肾组织中CD40 40L的原位表达特点。以期阐明CD40 CD40L共刺激通路在移植肾脏慢性排斥反应中的作用。方法 :共分 3个实验组 :即同种异体移植不用药对照组、同品系移植组、同种异体移植CSA治疗组。用SP免疫组织化学方法对移植肾脏组织CD40 CD40L表达进行观察 ,并结合肾间质中浸润的炎性细胞CD3+和CD68+细胞数进行分析。结果 :慢性排斥肾组织中CD40 CD40L表达分布不同 ,间质淋巴细胞以表达CD40为主 ,CD40L表达肾实质细胞表达为主。慢性排斥移植肾组织间质CD40 +细胞数与CD3+、CD68+细胞数密切相关 ;CD40L+细胞数与CD3+细胞数呈正相关 ,与CD68+细胞数无相关性。结论 :CD40 CD40L共刺激通路可能在移植肾慢性排斥的发生。 Objective:To study the characteristic of CD40 and CD40L expression during chronic rejection(CR) of renal allograft in rats and to investigate the possible pathogenic role of CD40/CD40L in CR allograft rejection Methods:Three groups of rats were included The expression of CD40/CD40L in the renal tissue was analyzed by immunochemistry of SP method The data was interpreted in the light of infiltrating CD3 and CD68 in the intersitium Results:There was different distribution of CD40 and CD40L in rat CR allograft rejection The striking CD40 staining of graft cellular inflitrates was observed in renal allografts with CR, CD40L expression predominantly presented in renal parenchyma of kidney allografts CD40 + and CD40L + cells in grafts of CR were significantly higher than those in the isograft group Furthermore,CD40 + infiltrates of renal allografts with CR had a positive correlation with CD3 +,CD68 + cells in the intersitium of grafts with CR CD40L + infiltrates also had a positive correlation with CD3 + infiltrates and had no correlation with CD68 + infiltrates of grafts with CR Conclusion:CD40/CD40L costimulatory molecules might play an important role in the pathogenesis of chronic renal allograft rejection
作者 周广臣 许纯孝 张怀强 关广骤 蒋小刚
机构地区 南京军区南京总医院解放军肾脏病研究所 山东医科大学第二附属医院泌尿外科
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2003年第1期49-53,共5页 Chinese Journal of Immunology
基金 山东省医药卫生科研项目 ( 1999A2 1)
关键词 肾移植 慢性排斥反应 共刺激分子 Kidney transplantation Chronic rejection Costimulatory molecule
分类号 R392.4 [医药卫生—免疫学]
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参考文献10

  • 1[1]Tullius S G, Hancock W W, Heemann U et al. Reversibility of chronic renal allograft rejection: critical effect of time after transplantation suggests both host immune dependent and independent phases of progressive injury [J] .Transplantation, 1994;58:93.
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同被引文献15

  • 1李宁,袁育康,吴军.腺病毒介导CTLA4Ig和Iκ-Bα双基因在ECV304细胞中的表达[J].细胞与分子免疫学杂志,2005,21(1):90-93. 被引量：1
  • 2Didier A, Mandelbrot MD, Mohamed H, et al. Role of novel T-cell costimulatory pathways in transplantation[J]. Cur Opin in Organ Transplant, 2003, 8(1): 25-33.
  • 3Sambrook J, Fritsch EF, Maniatis T. Molecular cloning[M]. 12nd ed. New York: Cold Spring Harbor Laboratory Press, 1989: 852-898.
  • 4Bachmann MF, Hunziker L, Zinkernagel RM, et al. Maintenance of memory CTL responses by T helper cells and CD40-CD40 ligand: antibodies provide the key[J]. Eur J Immunol, 2004, 34(2): 317-326.
  • 5Jin YZ, Xie SS. Bicistronic adenovirus-mediated gene transfer of CTLA4Ig gene and CD40Ig gene result in indefinite survival of islet xenograft[J]. Transplant Proc, 2003, 35(8): 3165-3166.
  • 6Kanaya K, Tsuchida Y, Inobe M, et al. Combined gene therapy with adenovirus vectors containing CTLA4Ig and CD40Ig prolongs survival of composite tissue allografts in rat model[J]. Transplantation, 2003, 75(3): 275-281.
  • 7Didier A M, Mohamed H S, Mohamed H, et al. Role of novel T-cell costimulatory pathways in transplantation[J]. Curr Opin Organ Transplant, 2003, 8(1): 25-33.
  • 8Sambrook J, Fritsch E F, Maniatis T, et al. Molecular cloning [M]. 2nd ed. New York: Cold Spring Harbor Laboratory Press, 1989. 852-898.
  • 9Bachmann M F, Hunziker L, Zinkernagel R M, et al. Maintenance of memory CTL responses by T helper cells and CD40-CD40 ligand: antibodies provide the key[J]. Eur J Immunol, 2004, 34(2): 317-326.
  • 10Jin Y Z, Xie S S. Bicistronic adenovirus-mediated gene transfer of CTLA4Ig gene and CD40Ig gene result in indefinite survival of islet xenograft[J]. Transplant Proc, 2003, 35(8): 3165-3166.

引证文献3

二级引证文献6

中国免疫学杂志
2003年 第1期

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