腺病毒载体介导的CD基因转移联合5-FC对大鼠卵巢癌细胞株的作用研究

Gene Therapy for Ovarian Cancer by Adenovirus-mediated Transduction of Cytosine Deaminase Gene in vitro

目的 研究胞嘧啶脱氨酶 (CD)基因转移及其与 5 -氟胞嘧啶 (5 - FC)联合应用对卵巢癌细胞株生长的影响。方法 用复制缺陷型重组腺病毒为载体 ,将 CD基因转染大鼠卵巢癌细胞株 NU TU - 19细胞 ,加入含 5 - FC的培养液培养。 MTT法检测培养孔吸光度值 ,计算细胞存活率。结果 5 - FC对转染了 CD基因的 NU TU - 19细胞的生长抑制作用显著增强 ,并随着腺病毒滴度和 5 - FC浓度的增加 ,该作用不断增强。此外 ,5 - FC可通过旁观者效应杀伤 CD基因转染细胞周围未转入该基因的 NU TU - 19细胞。结论 CD基因转移联合 5 - FC作用对 NUTU-

Objective To study the value of adenovirus mediated expression of the Escherichia coli cytosine deaminase (CD) gene with 5 fluorocytosine (5 FC) in the gene therapy of ovarian cancer cells. Methods Ovarian cancer cells were transduced CD gene by adenovirus vector and exposed to varying concentration of 5 FC. After 5 days of incubation, MTT assays were performed to measure the cell viability. Results It was shown that tumor cells transduced with CD gene had a high sensitivity to 5 FC. Mixed cellular assay revealed that CD postive cells had a neighbor cell killing effect on CD negative cells when exposed to 5 FC. 20% CD positive cells killed more than 80% mixed cells. Conclusion CD/5 FC gene therapy approach is an effective anti tumor strategy in the treatment of ovarian cancer.