大鼠下丘脑室旁核内AVP mRNA表达动物模型的建立

Immuno-responsiveness in Rats: The Establishment of Immune-enhancing and Immune-suppressing Animal Models

目的 建立免疫增强 /免疫抑制动物模型 ,以研究免疫应答不同时期大鼠下丘脑室旁核内精氨酸加压素神经元基因表达的动态变化。方法 采用多点、多部位、多方式注射牛血清白蛋白建立免疫增强动物模型 ;低剂量、隔日腹腔注射环磷酰胺 (CY)建立免疫抑制动物模型 ,并在不同时间点同时取动物的血清进行研究。结果 1初次抗原刺激后第 6 d血清 Ig G及 IL - 2的含量开始升高 ,再次刺激后第 6 d(实验的第 16 d)血清 Ig G及 IL - 2的含量达峰值 ;2第 1、2次腹腔注射 CY后血清 Ig G及 IL - 2的含量开始降低 ,4次注射 CY后血清 Ig G及 IL - 2的含量降至最低。结论 牛血清白蛋白和 CY是建立免疫增强和免疫抑制动物模型的较好试剂 ,牛血清白蛋白可同时直接增强体液免疫和细胞免疫功能 ,CY直接抑制细胞免疫功能 。

Objective To investigate the dynamic variation of AVP mRNA expression in the paraventricular nucleus of hypothalamus during immune responses in rats and further reveal the genetic modulating mechanisms by which nervous system modulates the immune function. Methods The multiple points, locations and ways of bull serum albumin (BSA) injection were used to establish immune enhancing animal models, and the lower doses and 1 day interval of CY intraperitoneal injection were used to establish immune suppressing animal models. In the different periods of the immune response, the serum and the brain of the same rat were taken to be tested at the same time. Results Six days after primary antigen stimulating the levels of IgG and IL 2 began to escalate, and 6 days after the antigen restimulating the IgG and IL 2 reached the highest level. After the first two CY injections the levels of IgG and IL 2 began to decline, and after 4 CY injections the IgG and IL 2 reached the lowest level. Conclusion BSA and CY are ideal agents for establishing immune enhancing and immune suppressing animal models. BSA could enhance both the humorous and cellular immune function directly. The CY could directly suppress the cellular immune function, but it could indirectly suppress the humorous immune function.