Bax蛋白在骺板软骨细胞中的表达

Expression of Bax Protein in the Cartilage Cells of Epiphyseal Plate

目的 研究凋亡相关基因 Bax在正常骺板软骨细胞中的表达。方法 采用免疫组化 ABC法。结果 Bax的免疫反应阳性物在正常骺板软骨的各个区中有不均衡的表达 ,在骺板软骨膜下的软骨细胞中没有 Bax表达 ;在骺板的静止区 Bax的免疫反应阳性物在软骨的细胞质中表达 ;在骺板的增殖区位于细胞质中的 Bax免疫阳性反应增强 ;在骺板的肥大区 Bax的免疫反应强度达到最大值 ,免疫反应阳性物位于软骨细胞的胞质和胞核中。结论

Objective This experiment was designed to investigate the expression of Bax in the chondrocytes of the epiphyseal plate cartilage. Methods Immunocytochemistry ABC method was used to determine the distribution of Bax immunoreactivity in the epiphyseal plate cartilage of rats. Results Bax immunoreactivity was non uniformly distributed throughout the epiphyseal cartilage. There was no Bax expression in chondrocytes under the perichondrium. Bax expression was observed in the resting chondrocytes of epiphysis; it increased in the proliferative chondrocytes of epiphysis, and immunoreactivity was mainly in the cytoplasm of chondrocytes; Bax expression was maximal in the hypertrophic chondrocytes of epiphysis, and immunoreactivity was mainly in the cytoplasm and nuclei of chondrocytes. Conclusion These immunocytochemical data indicate that Bax may promote chondrocytes apoptosis of epiphysis through an accumulative increase in quantity.