电沉积石墨烯的非共价功能化用于SPR核酸传感的协同增敏

Noncovalent functionalization of graphene for sensitizing SPR-based DNA sensing synergistically with biocatalytic polymerization

研究了一种高效的表面等离子共振(SPR)基DNA分析方法,利用非共价功能化的石墨烯纳米片作为基底,并以酶催化诱导的聚合作为质量中继。该策略有多方面的目标:首先,通过原位优化的石墨烯薄膜的电生成,该过程由原子力显微拓扑图表征,来敏化总体的SPR输出;其次,用于调制镍离子螯合的胺基三乙酸支架,其吸附在石墨烯支撑的苝基衍生物表面,上端生物素化捕获探针可以自组装,并保有一定的方向;最后,通过辣根过氧化物酶标记的报告单元,实时地将苯胺添加剂转化为聚苯胺沉淀,以协同实现信号的放大。运用上述配置,获得了一个对特异性DNA靶标精确且可重现传感的平台,检测下限达到飞摩尔水平,从而展现了以二维纳米材料为独特SPR基础设施的有益探索和开发。该"自下而上"的建构、与置顶"自上而下"的重量反应器,极有可能拓展并移植用于蛋白质的定量。

A highly efficient surface plasmon resonance (SPR )-based DNA assay is developed, by employing noncovalently functionalized graphene nanosheets as the substrate, and enzymatic catalysis-induced polymerization as the mass relay.The objective of this strategy was manifold:first of all, to sensitize the overall SPR output by in situ optimized electrogeneration of graphene thin-film,that was characterized by atomic force micro-topography;secondly,to regulate the self-assembly and orientation of biotinylated capture probes on nickel-chelated nitrilotriacetic acid scaffolds, which were anchored onto graphene-supported pyrenyl derivatives;and lastly, to synergize the signal amplification via real-time conversion of the additive aniline into polyaniline precipitation by horseradish peroxidase-tagged reporters. With this setup,a precise and replicable DNA sensing platform for specific targets was achieved with a detection limit down to femtomolar, demonstrating a beneficial exploration and exploitation of two-dimensional nanomaterials as unique SPR infrastructure.The possibility of such “bottom-up”architecture mounted with “top-down ” weight reactor would be most likely extensible and adaptable to protein determinations.