牛奶及奶粉中黄曲霉毒素M_1的快速测定

Rapid detecting aflatoxin M_1 in milk and milk powder

采用免疫亲和柱——荧光光度法快速测定了牛乳及乳制品中黄曲霉毒素M1。试样经过离心、脱脂、过滤后,滤液经过键合有黄曲霉毒素M1特殊抗体的免疫亲和柱净化,此抗体对黄曲霉毒素M1具有专一的识别能力,黄曲霉毒素M1键合在分离柱中的抗体上。用甲醇与水之比为10∶90的混合液将免疫亲和柱上杂质除去;以甲醇与水之比为80∶20的混合液通过分离柱洗脱;加入溴溶液衍生,以提高测定灵敏度。衍生化后的洗脱液于荧光光度计中测定黄曲霉毒素M1,检测低限为0.1μg/kg;在0.1～1.0μg/kg范围内,回收率为89.6%～96.1%,变异系数为0.52%～5.8%,分析一个样品的时间小于30min,分析过程中不使用黄曲霉毒素M1标准物质。结果表明,本方法具有准确、简单、快速、安全等优点,可以满足少量和批量样品的检测需要。

Aflatoxin M1 in milk and milk powder are extracted and separated by immunoaffinity column.Then the columns in washed with methanol water (10∶90) tw ice and the concentrated aflatoxin M1 are eluted with methanol water (90∶10).Th e aflatoxin M1 is determined by fluorometer with bromine.Test portions and spike d at 0.1,0.5,1.0 ìg/kg,recoveries are 89.6%～96.1%,variable coefficient are 0.52%～5.8%,the lowest.It is 30 minutes for analysis one sample.