巨细胞型胶质母细胞瘤临床病理、免疫组化和超微结构观察

Clinicopathological,immunohistochemical and ultrastructural observation on giant cell glioblastoma

目的 探讨巨细胞型胶质母细胞瘤的来源和病理诊断标准。方法 对 2 5例确诊病例进行临床病理观察 ,用 7种免疫组化试剂进行检测 ,并对其中 8例进行电镜观察。结果 2 5例GFAP、S 1 0 0和vimentin全部 (+)。超微结构特征是瘤细胞巨大 ,胞质宽阔 ,核大畸形 ,胞质内胶质丰富。结论 本瘤来源于星形细胞 ,属于胶质母细胞瘤的一个亚型。其病理组织学诊断标准为 :①具备胶质瘤的Ⅲ级改变 ;②异型瘤巨细胞数 >50 % ;

Objective To investigate the origin and pathological diagnostic criteria of giant cell glioblastoma (GCG). Methods A total of 25 cases of GCG were studied by clinicopathological analysis and immunohistochemical stains,in which 8 cases were observed under electron microscopy. Results All of them were positive for GFAP, S 100 protein and vimentin. The ultratructural features were that the bodies of tumor cells were huge with abundant cytoplasm in which rich glial fibers could be found. The nucleus was large and bizarre. Conclusions This tumor origins from astrocyte, and is a subtype of glioblastomas. The pathological diagnostic criteria are as follows: ① there are characteristics of giloma grade 3; ② the proprtion of giant tumor cells is more than 50%, and ③ immunohistochemical and ultrastructural studies showed that the tumor cells are astrocytic origin.