添加纤维素降解菌对牛粪堆肥特性的影响

EFFECT OF ADDING CELLULOSE DEGRADER ON THE CHARACTERISTICS OF CATTLE MANURE COMPOSTING

在实验室规模的牛粪和木薯杆堆肥中,筛选出能降解纤维素的混合菌,经在平板培养基中分离纯化和进一步筛选,得到对纤维素降解效果较好的纯菌株CE5,将其在液体培养基中富集后添加到相同的堆肥原料中,研究接种CE5对堆肥特性的影响。结果表明:接种CE5的试验组堆体温度在堆肥过程中均高于未接种对照组;堆肥结束时,实验组的C/N低于对照组,种子发芽指数、全氮和有效氮含量均高于对照组,水溶性有机碳和有机质含量相当,说明实验组的腐熟情况好于对照组,通过扫描电镜对堆肥结束后样品检测,可以看出试验组中木薯杆的降解效果优于对照组,证明接种CE5提高了有机肥的品质。

Mixed cellulose degraders were screened from the cattle manure and stalks of cassava composting in the laboratory,further isolation was done through alternative plating method,and a strain named CE5 was obtained,which could effectively degrade cellulose. The strain CE5 was enriched in liquid medium,and inoculated in the composting materials to assess the influence of the addition of CE5 on composting process. The results showed that adding CE5 had a higher temperature than controlling samples,at the end of composting,the germination index,total N and effective N of experimental samples were higher than control samples,while C /N was lower,WSC and organic matter had no significant difference,indicating that biodegradation of the experimental group was more than controlling samples. Also,SEM analysis revealed that stalks of cassava of experimental samples were better degraded than the controlling group. Hence,adding CE5 can enhance the quality of composts.