垃圾填埋场覆盖材料的甲烷氧化能力与甲烷氧化菌定量分析

QUANTITATIVE ANALYSIS OF METHANE OXIDATION CAPACITY AND METHANE OXIDIZING BACTERIA IN TYPICAL MSW LANDFILL COVERING MATERIALS

分别采用厌氧瓶培养方法和荧光定量PCR技术定量测试了6种典型填埋场覆盖材料的甲烷氧化能力和甲烷氧化菌数量,并分析了典型覆盖材料甲烷氧化能力与甲烷氧化菌含量及物料特性的相关关系。结果表明:厌氧填埋陈腐垃圾、准好氧填埋陈腐垃圾和老覆土的甲烷氧化速率约高于粪便堆肥和垃圾堆肥1个数量级,约高于新覆土2个数量级;厌氧和准好氧填埋陈腐垃圾的甲烷氧化菌含量约高于老覆土和粪便堆肥1个数量级,约高于垃圾堆肥和新覆土2个数量级。覆盖材料的甲烷氧化菌数、甲烷氧化速率与物料填埋或驯化时间呈极显著正相关(p<0.01);甲烷氧化菌数与覆盖材料的甲烷氧化速率、含水率、总氮呈显著性正相关(p<0.05);覆盖材料的甲烷氧化速率与其理化性质之间无明显相关性,而是与覆盖材料本身的甲烷氧化菌含量显著相关。

The methane oxidation capacity of 6 kinds of typical landfill cover materials was studies by anaerobic serum bottle culturing experiment. And real-time fluorescence quantitative PCR technology was used to determine the pmoA gene copies number of methanotrophs in the landfill cover materials. The results showed that the methane oxidation capacity and the abundance of methanotrophs has a significant positive correlation( p < 0. 05),and the methane oxidation capacity and the time of the materials exposed to the higher-content methane surrounding has a very significant positive correlation( p < 0. 01). In addition,higher-content methane surrounding will stimulate the methane oxidation capacity of the cover materials. The characteristics,such as moisture content of materials,organic matter content,pH,etc,compared with the time of the materials exposed to the higher-content methane surrounding, was not significant influence factors on the number of methanotrophs.