漆酶对水中氯酚类物质的去除

Removal of chlorophenols in aqueous solution by laccase

利用漆酶对2,5-二氯苯酚(2,5-DCP)、2,6-二氯苯酚(2,6-DCP)、2,4,5-三氯苯酚(2,4,5-TCP)、2,4,6-三氯苯酚(2,4,6-TCP)等4种物质进行去除研究,考察了温度、时间、pH及漆酶添加量对去除效果的影响,并基于最佳条件下的去除效果,探讨了底物结构对漆酶转化氯酚类物质的影响.结果表明,温度升高,去除率呈先升高后降低再升高的现象;pH增大,去除率有增加的趋势;漆酶添加量在一定范围内与去除率呈正相关,当添加量高于20%后,去除率降低;在较短时间(0.5 h)内,漆酶对4种氯酚类物质的去除均能达到较好的效果.最佳条件下,漆酶对2,5-DCP、2,6-DCP、2,4,5-TCP和2,4,6-TCP的去除率分别为86.2%、45.3%、98.7%、84.8%;氯原子取代位置与取代数目相比对去除效果具有更重要的影响.

In this study,laccase was applied to remove chlorophenol compounds including 2,5-dichlorophenol(2,5-DCP),2,6-dichlorophenol(2,6-DCP),2,4,5-trichlorophenol(2,4,5-TCP)and 2,4,6-trichlorophenol(2,4,6-TCP).The influence by temperature,time,pH and enzyme amount on the removal rate was investigated.Based on the optimal condition,the effect of substrate structure on the removal rate by laccase was also investigated.The results indicated that the removal rate of all compounds increased,then decreased with the increase of temperature.A further increase was observed while increasing the temperature.The removal rate increased with the increase of pH values.Laccase amount showed a positive influence on the removal rate when laccase amount was lower than 20%.The removal rate decreased when laccase amount was higher than 20%.Under the optimal condition,the removal rate was 86.2%,45.3%,98.7%and 84.8%for 2,5-DCP,2,6-DCP,2,4,5-TCP and 2,4,6-TCP within 0.5 h,respectively.In addition,the substitution position had a higher influence on the removal rate in comparison to the number of chlorine atoms.