不同基质条件下厌氧氨氧化反应器中硝化细菌的分离及选育异养AOB菌株的特性

Separation of nitrifying bacterium from anammox reactors with different substrates and characteristics of heterotrophic AOB

对长期分别以模拟配水和生活污水为基质运行的ASBR厌氧氨氧化反应器中的硝化细菌进行解析,对比了2种基质条件下厌氧氨氧化反应器中硝化细菌的群落结构,并分别选育的异养AOB菌株a-2-2和YA-2-4L的生长和脱氮特性进行研究。实验结果表明,以生活污水为基质的反应器比模拟配水为基质的反应器中硝化细菌的生态多样性更丰富。在生活污水为基质的反应系统中挑选的a-2-2菌株为假单胞菌属,其48 h内NH+4从39 mg/L下降到5.83 mg/L,NH+4去除率为85.06%,其最适生长pH为7.5,而在pH为8时具有最佳的硝化能力,在盐度为0%~4%范围内活性较好,其最适生长温度36℃,但在32℃具有最佳的硝化能力;在以模拟配水为基质的反应系统中挑选的YA-2-4L菌株为不动杆菌属,其48h内NH+4从39.1 mg/L下降到6.95 mg/L,NH+4去除率为82.22%,最适生长pH为7.5,而在pH为8时具有最佳的硝化能力,在盐度为0%~4%范围内活性较好,其最适生长温度为36℃,但在32℃具有最佳的硝化能力。

Nitrifying bacteria from anammox reactors with simulated wastewater and domestic sewage were studied respectively and the growth and nitrogen removal characteristics of heterotrophic AOB strains of a-2-2 and YA-2-4L were researched. Experimental results show that the ecological diversity of nitrifying bacterium in the reactor with domestic sewage was more abundant than the simulated wastewater. In domestic wastewater system, one heterotrophic AOB named a-2-2 which belongs to pseudomonas genus was got. NH+ 4content fell from 5. 83 mg/L to 39 mg/L. The removal rate of NH+ 4reached 85. 06% within 48 h. Its optimal growth pH was 7. 5,and the pH of 8 met the best nitrification ability. It had the best activity when the salinity was within the scope of 0% ~ 4%. Its optimal growth temperature was 36℃,but had the best nitrification ability at 32℃. In simulated wastewater system,another heterotrophic AOB named YA-2-4L which belongs to acinetobacter genus was got. NH+ 4content fell from 6. 95 mg/L to 39. 1 mg/L. The removal rate of NH+ 4reached 82. 22% within 48 h. The optimum growth pH was 7. 5,and the pH of 8 met the best nitrification ability. It had the best activity when the salinity was within the scope of 0% ~ 4%. Its optimal growth temperature was 36℃,but had the best nitrification ability at 32℃.