餐厨垃圾废液制备高效解磷菌肥

Preparation of liquid bacterial fertilizer of highly-effective phosphate-solubilizing bacteria from food waste-recycling wastewater

从小麦土中筛选出2株高效解磷菌P1和P7,其解磷能力在10 d内达到117.57 mg·L^(-1)和219.59 mg·L^(-1)。经16S rDNA测序鉴定及构建系统进化树,鉴定P1为Enterobacter cloacae,P7为Kosakonia cowanii。通过正交实验及单因素实验,对解磷菌在餐厨废液中生长条件进行了优化。结果表明,Kosakonia cowanii生长条件影响程度的顺序依次为温度>pH>摇床转速>接种量,最优的培养条件为温度25℃,pH=7,摇床转速140 r·min^(-1),接种量1.0%。将筛选的解磷菌制备成解磷菌肥,并进行大蒜盆栽实验。结果表明,30 d后,土壤有效磷相比空白提升了16.32%,大蒜叶宽与叶数相比空白分别提升了63.16%和60.00%。

Two highly-effective phosphate-solubilizing bacteria, named P1 and P7, were screened from wheat soil, and their phosphate-solubilizing abilities within 10 d reached 117.57 mg · L-1 and 219.59 mg · L-1,respectively. On the basis of 16 S rDNA sequencing and phylogenetic tree construction, P1 and P7 were identified as Enterobacter cloacae and Kosakonia cowanii, respectively. The orthogonal and single factor experiments were carried out to optimize the growth conditions of phosphate-solubilizing bacteria in the food waste-recycling wastewater. The results showed that the significance of factors affecting the growth conditions of Kosakonia cowanii bacteria was temperature>pH>shaking speed>inoculum concentration. The optimal culture conditions determined by orthogonal experiment were following: temperature of 25 ℃, pH of 7, shaking speed of140 r · min-1, and inoculum concentration of 1.0%. The phosphate-solubilizing bacteria fertilizer was prepared from the screened phosphate-solubilizing bacteria, and the pot experiments of cultivating garlic were conducted accordingly. The results showed that the available phosphorus in soil increased by 16.32% compared to control check after 30 days, and the width and number of garlic leaf increased by 63.16% and 60.00%, respectively.