利用米曲霉发酵餐厨垃圾产水解酶促进污泥厌氧消化

Promoted sludge anaerobic digestion by the hydrolase produced from food waste fermentation with Aspergillus oryzae

将米曲霉接种到餐厨垃圾中生产水解酶,并利用此生物酶强化污泥厌氧消化。对比分析了富含水解酶的餐厨垃圾(实验组)、中温灭活富含水解酶的餐厨垃圾(对照组A)和未发酵餐厨垃圾(对照组B)分别与剩余污泥厌氧共消化情况;考察了实验组对污泥厌氧体系的促进效果;并运用3种模型对反应体系中底物的产甲烷潜力进行了拟合。结果显示,实验组甲烷含量最高可达71.51%;挥发性固体单位累计甲烷产量为(308.46±19.47) mL·g^(-1),相比对照组A和对照组B显著提高(P<0.05),分别是对照组A和对照组B的1.56倍和1.31倍。修正的Gompertz模型优于一级动力学模型和Cone模型,能够很好地预测厌氧消化体系的最大甲烷产量,更适宜于拟合富酶餐厨与剩余污泥厌氧共消化体系。

Aspergillus oryzaewas inoculated into food waste for hydrolase production and this biological enzyme was then used to strengthen sludge anaerobic digestion.The comparison of sludge anaerobic co-digestions was performed among three different types of additives:food waste with hydrolase(experimental group),food waste with medium inactivated hydrolase(control group A)and raw food waste(control group B).The promotion efficiency of experimental group for sludge anaerobic digestion was evaluated.Three models were used to fit the methanogenic potential of the substrate in above reaction system.Results indicated that the highest methane content of experimental group was 71.51%.Its cumulative methane yield per unit of volatile solid was(308.46±19.47)mL·g-1,which presented better performance than other two control groups of A and B(P<0.05),and were1.56 times and 1.31 times of control group A and control group B,respectively.Comparing with the first-order kinetic model and Cone model,the modified Gompertz model was superior to predict the maximum methane production of the anaerobic digestion system,and more suitable for fitting the anaerobic co-digestion system including food waste enriched with enzyme and excess sludge.