摘要
Quorum sensing(QS)regulation of the composition of ammonia-oxidising archaea(AOA)and ammonia-oxidising bacteria(AOB)communities and functions in wastewater treatment was investigated.Specifically,we explored the role of N-acyl-L-homoserine lactones(AHLs)in microbial community dynamics in activated sludge.On average,the specific ammoniaoxidising-rate increased from 1.6 to 2.8 mg NH_4^+-N/g MLSS/hr after treatment with long-chain AHLs for 16 days,and the addition of AHLs to sludge resulted in an increased number of AOA/AOB amoA genes.Significant differences were observed in the AOA communities of control and AHL-treated cultures,but not the AOB community.Furthermore,the dominant functional AOA strains of the Crenarchaeota altered their ecological niche in response to AHL addition.These results provide evidence that AHLs play an important role in mediating AOA/AOB microbial community parameters and demonstrate the potential for application of QS to the regulation of nitrogen compound metabolism in wastewater treatment.
Quorum sensing(QS)regulation of the composition of ammonia-oxidising archaea(AOA)and ammonia-oxidising bacteria(AOB)communities and functions in wastewater treatment was investigated.Specifically,we explored the role of N-acyl-L-homoserine lactones(AHLs)in microbial community dynamics in activated sludge.On average,the specific ammoniaoxidising-rate increased from 1.6 to 2.8 mg NH_4^+-N/g MLSS/hr after treatment with long-chain AHLs for 16 days,and the addition of AHLs to sludge resulted in an increased number of AOA/AOB amoA genes.Significant differences were observed in the AOA communities of control and AHL-treated cultures,but not the AOB community.Furthermore,the dominant functional AOA strains of the Crenarchaeota altered their ecological niche in response to AHL addition.These results provide evidence that AHLs play an important role in mediating AOA/AOB microbial community parameters and demonstrate the potential for application of QS to the regulation of nitrogen compound metabolism in wastewater treatment.
基金
supported by the National Natural Science Foundation of China (No. 41501250)
the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDB15030101)
the National Key Research and Development Program of China (Nos. 2016YFC0500401 and 2017YFC050580301)
