用于1,3-二硝基苯快速检测的免疫传感器研究

Immunosensor for Rapid Detection of 1,3-Dinitrobenzene

利用倏逝波全光纤生物传感平台,发展了一种用于1,3-二硝基苯快速检测的免疫传感器,其检测限可达0.054 mg·L-1,检测时间少于10 min.采用先将小分子污染物与惰性蛋白OVA结合制备成复合物,然后将该复合物通过双功能试剂连接到硅烷化后的光纤探头表面作为生物识别元件,使得传感器具有良好的鲁棒性和再生性能,可以重复使用上百次,而且没有明显的活性损失.实际水样的加标回收实验表明,所有样品的加标回收率在80%~120%之间,相对标准偏差为4.5%~10.0%,具有很好的精密度和准确性,受环境基质的影响较小,因此,该免疫传感器能够用于实际水样中1,3-二硝基苯的快速检测.

An immunosensor for the rapid detection of 1,3-dinitrobenzene was developed based on an evanescent wave all-fiber biosensing platform with the detection limits of 0. 054 mg·L- 1,and the detection cycle was less than 10 min. Hapten-carrier conjugates NB-OVA were synthesized by mixing 4-nitrohippuric acid and OVA activated by EDC,and then the conjugates were immobilized onto the silane layer on the probe with a heterobifunctional crosslinker. The probe modified had good robustness and regeneration performance,which allowed the performance of more than 100 assay cycles without significant loss of reactivity. Several water samples of different origins were measured with less than 4. 5%-10. 0% deviation of the detection and the recovery rate of 1,3-dinitrobenzene was between 80% and 120%,which proved the system's precision and accuracy and negligible matrix effects. This immunosensor shows great potential in rapid detection of 1,3-dinitrobenzene in practical waters.