常温低基质下碱度和溶解氧对厌氧氨氧化的影响

Influence of Alkalinity and DO on ANAMMOX Bioreactor at Normal Temperature and Low Substrate Concentration

通过改变进水碱度、光照条件和溶解氧,对以陶粒为填料的常温低基质上向流厌氧氨氧化生物滤柱的脱氮性能进行了研究.借助克隆测序技术对滤柱内的微生物菌群进行了分析.结果表明,水温为20℃±2℃,水力停留时间为3 h条件下,当进水碱度在44~350 mg·L-1时,随着碱度的降低,氨氮去除率从97.2%降至75.6%,总氮去除率从89.7%降至75.1%,而亚硝氮去除率稳定在99.7%,进水碱度为0 mg·L-1时,出水亚硝氮浓度突跃至4.9 mg·L-1.当进水碱度恢复至350 mg·L-1,存在自然光照情况下,氨氮去除率较没有光照时的氨氮去除率降低12.3%.水力停留时间为1.5 h条件下,当进水DO小于3mg·L-1时,平均氨氮去除率和亚硝氮去除率分别为99.7%和100%,平均总氮去除负荷为1.0 kg·(m3·d)-1.16S rRNA克隆测序鉴定结果表明,滤柱下部的厌氧氨氧化菌种类为Candidatus Jettenia asiatica和Candidatus Brocadia sp.,这两种菌对20℃±2℃水温有很好的适应性.

A lab-scale up-flow ANAMMOX bioreactor with ceramics as biomass carrier was started up. The influence of alkalinity and dissolved oxygen on ANAMMOX reaction at normal temperature and low substrate concentration was investigated. The results showed that, at (20 ± 2)℃ and an HRT of 3 h, when the alkalinity was between 44 mg·L - 1 and 350 mg·L - 1 , the ammonia removal efficiency was decreased from 97. 2% to 75. 6% and the TN removal efficiency was decreased from 89. 7% to 75. 1% as the alkalinity reduced. Meanwhile, the nitrite removal efficiency was stabilized at 99. 7% . When the alkalinity was 0 mg·L - 1 , the effluent nitrite concentration was increased to 4. 9 mg·L - 1 . Ammonia removal efficiency was decreased by 12. 3% because of light. When the HRT was 1. 5 h, the DO value was 《 3 mg·L - 1 , the average removal efficiencies of ammonia and nitrite were 99. 7% and 100% , respectively, the nitrogen removal rate was 1. 0 kg·(m3·d) - 1 . 16S rRNA phylogenic analysis was applied to analyze the microbial community structure. Results revealed that Candidatus Jettenia asiatica and Candidatus Brocadia sp. were adapted to normal temperature.