微生物除臭剂的筛选、复配及其除臭条件的优化

Screening,Combination of Microbial Deodorizer and the Optimization of Its Deodorizing Conditions

为了获取作为除臭微生物制剂的候选菌株,从垃圾渗滤液中分离筛选了4株具有对NH3和H2S高效降解菌株,分别标记为CC3、CC7、CC13和CC16.通过形态、生理生化和16S r DNA序列分析,分别鉴定为乳酸片球菌(Pediococcus acidilactici)、巨大芽孢杆菌(Bacillus megaterium)、嗜酸乳杆菌(Lactobacillus acidophilus)、粪产碱杆菌(Alcaligenes faecalis).菌株CC7、CC13和CC16组成的复配组合除臭效率最优,其复配比例为1∶1.5∶0.5,对NH3和H2S的去除率分别为83.56%和70.25%.通过单因素实验,确定微生物除臭剂最佳除臭条件:除臭时间为60 h,菌剂使用量为5%,除臭温度为30℃,初始培养基p H值为6.5.

In order to obtain the candidate strains of deodorizing microorganism,three optimization strains with relatively higher deodorizing capacity were isolated from landfill leachate,which were given the labels of CC3,CC7,CC13 and CC16. According to the results of morphological observation,physiological and biochemical tests,16 S r DNA sequence homology analysis,the strains CC3,CC7,CC13 and CC16 were identified as Pediococcus acidilactici,Bacillus megaterium,Lactobacillus acidophilus and Alcaligenes faecalis respectively. When the strains CC7,CC13 and CC16 were mixed with the inoculation rate was 1∶ 1. 5∶ 0. 5,the removal rates of ammonia and hydrogen sulfide were 83. 56% and 70. 25%. The optimal conditions of deodorizing by single factor test were deodorization time of 60 hours,sausage of 5%,temperature of 30℃,initial p H of 6. 5.