基于平面波导型荧光免疫传感器的双酚A检测适用性研究

Applicability of Bisphenol A Detection by a Planar Waveguide Fluorescent Biosensor

双酚A是一种环境内分泌干扰物,逐渐受到国内外广泛关注.平面波导型荧光免疫传感器可以对水样中存在的痕量双酚A进行快速高灵敏度检测.在最优检测条件下测得双酚A标准曲线的检出限为(0.04±0.007)μg·L-1,线性区间为0.16~22.40μg·L-1,半抑制浓度为(1.67±0.47)μg·L-1.加入0.5%的EDTA到样品溶液中可以削弱水体硬度的干扰,并在最优条件下测得4种实际水样的加标回收率在88%~111%之间,相对标准偏差小于15%,表明该方法可以运用于实际水样中双酚A的检测.

As a kind of environmental endocrine disruptors,bisphenol A received a wide attention around the world. The planar waveguide fluorescent biosensor can rapidly and sensitively detect traceable bisphenol A in water samples. Under the optimized test conditions,the typical calibration curve for BPA determination by the biosensor showed a detection limit of( 0. 04 ± 0. 007) μg·L-1.The linear response ranged from 0. 16 μg·L-1to 22. 40 μg·L-1. The 50% inhibition concentration( IC50) was( 1. 67 ± 0. 47)μg·L-1. By means of adding 0. 5% EDTA to sample solutions could weaken the interference of water hardness. Under the optimal conditions,the recovery ratios for four kinds of real water samples were in the range of 88% to 111%,with R. S. D. less than 15%.The results indicate that this method features the potential in the practical detections of bisphenol A in real water samples.