人工湿地空气微生物群落碳源代谢特征

Carbon Sources Metabolic Characteristics of Airborne Microbial Communities in Constructed Wetlands

采用BIOLOG-GN微平板分析自由表面流人工湿地空气微生物群落碳源代谢特征,阐明空气微生物群落代谢功能与环境因子相关性.结果表明,不同季节人工湿地空气微生物AWCD值、优势群落及Mc Intosh指数均存在差异,春季和夏季空气微生物碳源代谢强度明显高于秋季和冬季(P<0.01);4个季节人工湿地空气微生物优势群落依次为羧酸类代谢群落、糖类代谢群落、聚合物类代谢群落和羧酸类代谢群落,夏季空气微生物对6类化合物的利用水平高于其他季节(P<0.05).主成分分析显示,秋季和冬季空气微生物碳源代谢特征相似,但与春季和夏季差别较大,胺类是区分人工湿地夏季与春季、秋季和冬季的主要碳源类型,羧酸类是区分春季与秋季和冬季空气微生物代谢差异的主要碳源类型.不同季节人工湿地空气微生物群落结构主导影响因子存在差异.

Using BIOLOG-GN plates, this article describes the carbon sources metabolic characteristics of airborne microbial communities in a free surface-flow constructed wetland in different seasons and clarify the correlation between airborne microbial metabolic functions and environmental factors. The average well color development( AWCD),carbon metabolic profiles and Mc Intosh values of airborne microbial communities in different seasons were quite different. Analysis of the variations showed that AWCD in spring and summer differed significantly from that in autumn and winter( P < 0. 01). In the same season,the degree of utilization of different types of carbon by airborne microbes was different. Summer had a significant difference from other seasons( P < 0. 05).Dominant communities of airborne microbes in four seasons were carboxylic acids metabolic community,carbohydrates metabolic community,polymers metabolic community and carboxylic acids metabolic community respectively. Principal component analysis showed that the carbon metabolic characteristics of airborne microbial community in autumn were similar to those in winter but different from those in spring and summer. The characteristics of carbon metabolism revealed differences between summer and spring,autumn,or winter. These differences were mainly caused by amines or amides while the differences between spring and autumn or winter were mainly caused by carboxylic acids. Environmental factors,including changes in wind speed,temperature,and humidity acted to influence the carbon sources metabolic properties of airborne microbial community. The dominant environmental factors that acted to influence the carbon sources metabolic properties of airborne microbial community varied between different seasons.