CdSe/ZnS量子点对斑马鱼胚胎发育的毒性效应

Toxic Effects of CdSe/ZnS QDs to Zebrafish Embryos

本研究以模式生物斑马鱼(Danio rerio)为对象,观察在不同浓度Cd Se/Zn S量子点(Cd Se/Zn S QDs)暴露下,斑马鱼胚胎形态发育、氧化应激以及金属硫蛋白MT基因和应激蛋白Hsp70基因表达变化情况.结果表明,Cd Se/Zn S QDs对斑马鱼胚胎72 hpf(hours post fertilization)的半致畸效应浓度(EC50)为316.994 nmol·L-1.QDs暴露影响了斑马鱼胚胎的死亡率、畸形率、孵化率、自主运动频率和体长,以及引起胚胎卵凝集,心包囊肿,脊椎弯曲等多种毒性效应;同时导致斑马鱼体内超氧化物歧化酶(SOD)活性变化以及丙二醛(MDA)含量增加.QDs还诱导斑马鱼MT基因和Hsp70基因表达上调,斑马鱼机体产生一系列自我保护反应来减轻QDs所造成的伤害.这表明:Cd Se/Zn S QDs对斑马鱼胚胎产生了毒性效应,其毒性可能与其核心Cd2+的释放、粒径大小以及氧化应激有关.

The toxic effects of Cd Se / Zn S QDs on zebrafish( Danio rerio) embryos at different developmental stages were investigated in this study. The voluntary movement frequency,body length,hatching rate,mortality and malformation rate,SOD activities,MDA contents,mRNA expression of metallothionein( MT) and heat stress protein 70( Hsp70) were used as indicators. The results showed that the EC50 was 316. 994 nmol·L- 1for zebrafish embryos( 72 hpf) when exposed to Cd Se / Zn S QDs. After the Cd Se / Zn S QDs exposure,the embryos showed a significant increase in mortality and malformation rate,a decrease in hatching rate and body length,an advance in hatching time,and a changing in the spontaneous movement frequency,and many other toxic effects,such as the condensation of embryonic eggs,the formation of pericardial cysts and curvature of the spine. Moreover,it was found that the MDA contents in the embryos in Cd Se / Zn S QDs groups were significantly increased,and the SOD activities were changed. In addition,the mRNA expression level of MT and Hsp70 were up-regulated. All the information suggests that exposure of Cd Se / Zn S QDs can cause toxic effects on zebrafish embryos,and the effects may be related to the releasing of Cd2 +,particle size and oxidative stress.