耐高浓度沼液产油小球藻的分离鉴定与特征分析

Isolation, Identification and Characteristic Analysis of an Oil-producing Chlorella sp. Tolerant to High-strength Anaerobic Digestion Effluent

本研究从长期在空气中放置的沼液中分离得到1株可以耐受高浓度沼液的藻株,经形态和分子生物学方法鉴定为小球藻属的一种,命名为Chlorella sp.BWY-1.本研究所用的沼液来自于以固液分离后的猪场废水为发酵原料的沼气工程.与普通小球藻Chlorella regularis(FACHB-729)的对比研究表明,Chlorella sp.BWY-1在BG11和不同浓度的沼液中都有相对较强的生长速率、生物量积累能力和氮磷等污染物去除能力.Chlorella sp.BWY-1在BG11中有最高的生长速率和生物量生产力(324.40 mg·L-1,以dw计),但是其含油量和油脂生产力随着沼液浓度的增加而增加.在未稀释的沼液中Chlorella sp.BWY-1的含油量可达44.43%,油脂生产力达108.70 mg·L-1.分析结果表明该藻株在养殖废水处理和生物能源方面具有一定的应用潜力,可以结合固液分离、厌氧发酵等其他技术用于养殖场废水的处理和生物柴油的制取.

A Chlorella strain tolerant to high-strength anaerobic digestion effluent was isolated from the anaerobic digestion effluent with a long-term exposure to air.The strain was identified as a Chlorella by morphological and molecular biological methods,and named Chlorella sp.BWY-1.The anaerobic digestion effluent used in this study was from a biogas plant with the raw materials of swine wastewater after solid-liquid separation.The Chlorella regularis( FACHB-729) was used as the control strain.The comparative study showed that Chlorella sp.BWY-1had relatively higher growth rate,biomass accumulation capacity and pollutants removal rate in BG11 and different concentrations of anaerobic digestion effluent.Chlorella sp.BWY-1 had the highest growth rate and biomass productivity( 324.40 mg·L- 1) in BG11,but its lipid productivity and lipid content increased with the increase of anaerobic digestion effluent concentration.In undiluted anaerobic digestion effluent,the lipid productivity and lipid content of Chlorella sp.BWY-1 were up to44.43% and 108.70 mg·L- 1,respectively.Those results showed that the isolated algal strain had some potential applications in livestock wastewater treatment and bioenergy production,it could be combined with a solid-liquid separation,anaerobic fermentation and other techniques for processing livestock wastewater and producing biodiesel.