水介质中C_(60)纳米晶体颗粒与Cu^(2+)对小鼠腹腔巨噬细胞的复合毒性研究

Coupled Cytotoxicity of C_(60) Nano-Crystal Particle with Cu^(2+) to the Mouse Peritoneal Microphage RAW 264.7

为考察C60纳米晶体颗粒和Cu2+复合体系的生物毒性,本文以小鼠腹腔巨噬细胞RAW264.7作为受试对象,研究了C60纳米晶体颗粒物(n C60)和Cu2+对RAW 264.7的复合毒性影响.结果表明,当单独暴露n C6024 h,n C60浓度为6.6 mg·L-1时,细胞活性降低了45%,n C60浓度为9.9 mg·L-1时,细胞活性降低了70%,表现出剂量-毒性效应和时间-毒性效应.水中出现Cu2+可以降低n C60的细胞毒性,当Cu2+浓度为2 mg·L-1,n C60浓度为6.6 mg·L-1时,细胞的活性降低至25%,当Cu2+浓度为5 mg·L-1,n C60浓度为9.9 mg·L-1时,细胞的活性下降至15%.研究表明,n C60表面能够吸附Cu2+,并且吸附符合Langmuir吸附等温模型.n C60纳米晶体颗粒表面对Cu2+的吸附,可能是n C60对细胞毒性降低的重要原因.

This work evaluated the cytotoxicity effect of n C60nano-crystal particle associated with Cu2 +by using mouse peritoneal microphage RAW264.7 as the test cell line.The results showed that when exposed to n C60 of 6.6 mg·L- 1and 9.9 mg·L- 1for 24 h,the cell viability decreased 45% and 70%,respectively,and had obvious time-response and dose-response.It was found that Cu2 +could reduce the toxicity of n C60 on RAW 264.7.The cell viability reduced to 25% when exposed to 6.6 mg·L- 1n C60 with 2 mg·L- 1Cu2 +,and reduced to 15% when exposed to 9.9 mg·L- 1n C60 with 5 mg·L- 1Cu2 +.The n C60 could adsorb Cu2 +and the adsorption isotherm was fitted to the Langmuir adsorption isotherm.The adsorption of Cu2 +on the surface of n C60 may decrease the cytotoxicity n C60 on RAW 264.7.