水体氮磷营养盐水平与氯霉素浓度复合水体对苦草的生理生化影响

Physiological Effect of Vallisneria natans Under Different Concentrations of Nitrogen,Phosphorus and Chloramphenicol

通过实验室静态模拟,研究了在不同氮磷营养水平(中营养组、富营养组和超富营养组)和不同浓度(0、0.05、0.1、0.2μg·L-1)氯霉素复合水体中暴露7 d后,苦草叶片中过氧化物酶(POD)和超氧化物歧化酶(SOD)的活力以及丙二醛(MDA)、可溶性蛋白和叶绿素含量的响应.结果表明,富营养和0.2μg·L-1氯霉素组复合水体中,苦草的可溶性蛋白含量显著增加,其浓度为同一营养组中0μg·L-1氯霉素组的2.38倍.POD活力显著降低,富营养和0.2μg·L-1氯霉素组复合水体中活力降为同一营养组中0μg·L-1氯霉素组的33.84%.对SOD的影响随二者联合浓度增大而减小,中营养和0.2μg·L-1氯霉素组复合水体的联合作用对苦草SOD的抑制最大,其活力为同一营养组中0μg·L-1氯霉素组的28.59%.

The effects of peroxidase( POD),superoxide dismutase( SOD) activities and malondialdehyde( MDA),soluble proteins and chlorophyll in the leaves of Vallisneria natans exposed to different concentrations of nitrogen and phosphorus in the eutrophication water body and chloramphenicol after 7 days were investigated in the study. The soluble protein content increased significantly in group eutrophic water and 0. 2 μg·L- 1chloramphenicol,and the concentration of protein was 2. 38 times of that in group 0 μg·L- 1chloramphenicol. In group of eutrophic water and 0. 2 μg·L- 1chloramphenicol,POD activities decreased significantly to 33. 84% of that in group 0 μg·L- 1chloramphenicol. With the increasing of the joint concentration,SOD activities decreased. SOD activities in group of mesotrophic and 0. 2 μg·L- 1chloramphenicol was 28. 59% of that in group of 0 μg·L- 1chloramphenicol.