污水管网中无机氮类营养盐迁变规律

Transformation of Nitrogen Nutrients in the Urban Sewage Pipe Network

以一套长1 200 m的城市污水模拟管网为对象,采用人工配水方式,研究了管网中含氮化合物的变化情况,分析评价了沿程氮类营养盐的迁变转化特性.结果表明,以氯化铵为氮源基质,管网微生物将其同化合成生命所需的物质以进行细胞增殖.游离氨基酸、结合氨基酸、核酸是代谢产生的主要含氮有机化合物,其中氨基酸占溶解性有机氮(DON)的绝大多数.同时利用三维荧光光谱、尺寸排阻色谱技术对水中有机物的荧光特性及相对分子质量分布的表征结果显示,污水中有机物的特征荧光峰有所增多,主要以类蛋白、类微生物代谢产物为主,且荧光强度随管网沿程逐渐增强;水中的小分子营养盐在微生物的同化作用下转化为复杂的大分子有机物.

The paper focuses on the changes of nitrogen compounds in the Pipe network. A 1. 2km long urban sewage simulation network with artificial water distribution was selected as the research subject. Then we analyzed and evaluated the change characteristics of nitrogen nutrients along the pipe. The results proved that ammonium chloride as the nitrogen source matrix,which was necessary for microorganisms in the Pipe to survive and proliferate. Free amino acids,combined amino acids and nucleic acid produced by metabolism were the major nitrogenous organic compounds. Among which,amino acids accounted for dissolved organic nitrogen( DON) in the majority. The characterization results of three-dimensional fluorescence spectroscopy and size exclusion chromatography showed that the characteristic fluorescence peak of organic matter in waste water was increased,and the protein( tyrosine,tryptophan)-based fluorescence intensity gradually increased along the pipe. It was also found that small molecules of nutrients in the water were converted to complex organic molecules under the assimilation of microorganisms.