ANAMMOX菌利用零价铁转化氨和硝酸盐实验

Simultaneous Biotransformation of Ammonium and Nitrate via Zero-Valent Iron on Anaerobic Conditions

研究在自养条件下,零价铁促使厌氧氨氧化菌同步转化硝酸盐和氨氮的性能.添加零价铁,温度35℃±0.5℃,进水p H7~8,进水氨氮、硝态氮分别为50~100 mg·L^(-1)、50~100 mg·L~(^(-1))条件下,添加ANAMMOX菌后硝酸盐的还原加快8.2倍,并且出现硝酸盐和氨的同步转化,其转化速率最高达17.2 mg·(L·h)^(-1).改变反应时间及进水n(NH+4):n(NO-3),两者摩尔转化比于1.2~3.5范围内波动,该反应并非基元反应.实验证明,氨与硝酸盐同步转化途径为零价铁作用首先将硝酸盐还原为亚硝酸盐,生成的亚硝酸盐再与氨发生厌氧氨氧化反应.

Zero-valent iron( ZVI) was used to improve the biological autotrophic denitrification process between nitrate and ammonia by anaerobic ammonia oxidation( ANAMMOX) bacteria. With the addition of ZVI,the biological autotrophic denitrification process could be reacted in the influent condition of p H was 7-8,at 35℃ ± 0. 5℃,the concentration of ammonia was 50-100 mg·L^(-1)and the concentration of nitrate was 50-100 mg·L^(-1). The highest conversion rate could be reached to 17. 2 mg·( L·h)^(-1). With the change of reaction time and the molar ratio of nitrate and ammonia in influent,the final molar conversion ratio of nitrate and ammonia in effluent fluctuated between 1. 2-3. 5. The result showed that this autotrophic denitrification process was not belonged to elementary reaction.The mechanism of this autotrophic denitrification process could be summarized that with the reduction of ZVI,the nitrate could be reduced to nitrite. Hereafter,the ANAMMOX process reacted between the nitrite and ammonia.