ABR除碳-亚硝化耦合厌氧氨氧化处理城市污水

ABR Decarbonization-Nitrosation Coupled with ANAMMOX to Treat Municipal Wastewater

为推进厌氧氨氧化技术应用于城市污水处理,耦合亚硝化系统和厌氧氨氧化系统,并在其前端添加ABR除碳系统,构建ABR除碳-亚硝化耦合厌氧氨氧化工艺进行城市污水脱氮除碳,采用MiSeq高通量测序技术分析污泥中微生物菌群结构的变化情况.结果表明,ABR除碳系统出水COD平均浓度120 mg·L^(-1),不会对后续亚硝化系统和厌氧氨氧化系统产生不利影响,控制亚硝化系统出水和ABR除碳出水比例为2∶1作为厌氧氨氧化系统进水,满足ANAMMOX所需NO_2^--N和NH_4^+-N基质比1∶1左右的要求.一体式反应器总氮去除率在86%～92%,出水COD浓度在20～40 mg·L^(-1).同时,实验后亚硝化系统中与反硝化作用密切相关的γ-Protebacteria纲有所增加,厌氧氨氧化系统中具有较高微生物生长速率和增强脱氮速率功能的Sphingobacteria纲显著增加,ABR除碳-亚硝化耦合厌氧氨氧化工艺能够有效用于处理城市污水脱氮除碳.

In order to promote the application of anaerobic ammonium oxidation(ANAMMOX)to treat municipal wastewater,nitrosation was coupled with ANAMMOX and an ABR decarbonization system at the front of it;then used to build a system in which ABR decarbonization-nitrosation coupled with ANAMMOX was used to treat municipal wastewater.The high throughput sequencing technology of MiSeq was employed to analyze the structure of the microbial community in the sludge.Results showed that the average effluent COD concentration of the carbon removal system was 120 mg·L-1 and that the subsequent nitrosation system and ANAMMOX system would not be adversely affected by the effluent COD.Controlled by matching the nitrosation effluent with that from the ABR carbon removal system 2∶1,the ANAMMOX influent,kept the ratio of the NO2--N and NH4+-N matrix to ANAMMOX at about 1∶1.The total nitrogen removal rate of the integrated reactor was 86%-92%and the COD of the effluent was 20-40 mg·L-1.After the experiment,the number of bacteria in classγ-Proteobacteria in the nitrosation system,which are closely related to denitrification,increased.Members of class Sphingobacteria,which have the function of enhancing the growth rate of microorganisms and enhancing the rate of denitrification,were significantly increased in the ANAMMOX system.The nitrogen and carbon in the municipal wastewater could be stably and efficiently removed by the ABR Decarbonization-Nitrosation coupled with ANAMMOX process.