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重金属铅、铬胁迫对斜生栅藻的生长、光合性能及抗氧化系统的影响 被引量:30

Effects of lead and chromium on the growth,photosynthetic performance,and antioxidant activity of Scenedesmus obliquus
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摘要 以斜生栅藻(Scenedesmus obliquus JNU49)为研究对象,研究了重金属Pb2+、Cr3+、Cr6+对斜生栅藻生物量积累、光合性能及抗氧化系统的影响.结果表明,Pb2+、Cr3+、Cr6+对斜生栅藻的半数抑制浓度(IC50)分别为82.94、121.15、23.65μmol·L-1.半数抑制浓度胁迫下,斜生栅藻的放氧速率分别在第2~8 d(Pb2+胁迫)、1~3 d(Cr3+、Cr6+胁迫)受到明显抑制,而呼吸速率无明显变化;Pb2+胁迫下,斜生栅藻光系统Ⅱ(PSⅡ)的原初光能转化效率(Fv/Fm)、实际光能转化效率(Yield)、相对电子传递速率(rETR)均明显低于对照,光化学淬灭(qP)从第1~5 d高于对照,而第6~8 d则显著低于对照;Cr3+和Cr6+胁迫对斜生栅藻光合性能的影响不如Pb2+胁迫明显.斜生栅藻丙二醛(MDA)含量在重金属胁迫下变高,以第3、4 d尤为明显;超氧化物歧化酶(SOD)活性升高,于第3、4 d较为明显;过氧化氢酶(CAT)活性、总抗氧化活性(T-AOC)在重金属胁迫下均小幅度提升.通过本研究发现,斜生栅藻的生物量、光合速率、叶绿素荧光参数和SOD活性等指标对重金属胁迫的反应均较为灵敏,可作为评估重金属胁迫对微藻危害的指标. The influence of three heavy metals( Pb2+,Cr3+and Cr6+) on the growth,photosynthetic performance,and antioxidant system of Scenedesmus obliquus( JNU49) were investigated. The results showed that the IC50of Pb2+,Cr3+and Cr6+were 82. 94 μmol·L-1,121. 15 μmol·L-1and 23. 65μmol·L-1,respectively. Under the stress of IC50,the oxygen evolution rates of JNU49 were significantly inhibited from 2rdto 8thday under Pb2+stress,from 1stto 3rdday under the stress of Cr3+and Cr6+; however,the respiration rates were not obviously affected. Under the stress of Pb2+,Fv/Fm,Yield and rETR of PSⅡin JNU49 were obviously lower than the control,while qP was higher than the control from 1stto 5thday,and then became lower than the control from 6thto 8thday; photosynthetic performance of JNU49 was less influenced by the stress of Cr3+and Cr6+than that of Pb2+. Under the stress of heavy metals,MDA content of JNU49 increased significantly,especially on 3rdand 4thday; the activity of SOD increased obviously on 3rdand 4thday;while the activity of CAT and T-AOC of JNU49 increased slightly as well. This study suggested that biomass concentration,photosynthetic rate,chlorophyll fluorescence parameters and SOD activities of S. obliquus were sensitive to the stress of heavy metals. These parameters could be taken as indicators when evaluating the detriment of heavy metals stress towards microalgae.
出处 《环境科学学报》 CAS CSCD 北大核心 2014年第6期1606-1614,共9页 Acta Scientiae Circumstantiae
基金 国家自然科学基金(No.31170337) 国家高技术研究发展计划项目(No.21013AA065805) 中央高校基本科研业务费专项资金(No.21612326)~~
关键词 斜生栅藻 重金属胁迫 IC50 光合性能 抗氧化系统 Scenedesmus obliquus heavy metal stress IC50 photosynthetic performance antioxidant activity
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