钙和铅相互作用对鲫鱼毒性效应的影响

Effect of interaction between calcium and lead on the toxicity of crucian

以鲫鱼为材料,研究了钙和铅相互作用对鲫鱼毒性效应的影响.试验分为5组,分别为空白对照Ⅰ组、单独染铅Ⅱ组(0.1 mg·L-1)及铅钙联合组(Ⅲ组:0.1 mg·L-1铅+2 mmol·L-1钙,Ⅳ组:0.1 mg·L-1铅+4 mmol·L-1钙,Ⅴ组:0.1 mg·L-1铅+6 mmol·L-1钙),处理35 d后,检测鲫鱼肌肉、鳃中铅含量,以及肝胰脏中总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量.结果表明,与Ⅰ组比较,Ⅱ、Ⅲ组鲫鱼肌肉、鳃中铅含量增高(p<0.05),Ⅴ组鲫鱼肌肉中铅含量降低(p<0.05),与Ⅱ组比较,Ⅳ、Ⅴ组鲫鱼肌肉、鳃中铅含量降低(p<0.05);与Ⅰ组比较,Ⅱ组CAT活性降低(p<0.05),Ⅲ、Ⅳ和Ⅴ组CAT活性升高(p<0.05),与Ⅱ组比较,Ⅳ、Ⅴ组CAT活性升高(p<0.05);与Ⅰ组比较,Ⅱ、Ⅲ和Ⅴ组T-AOC活性降低(p<0.05),与Ⅱ组比较,Ⅲ、Ⅳ和Ⅴ组T-AOC活性增高(p<0.05);与Ⅰ组比较,Ⅱ、Ⅲ、Ⅳ和Ⅴ组MDA含量升高(p<0.05),与Ⅱ组比较,Ⅲ、Ⅳ和Ⅴ组MDA含量降低(p<0.05).由此可见,钙可竞争鲫鱼对铅的吸收,降低铅在其肌肉、鳃中的蓄积,提高鲫鱼肝胰脏的抗氧化能力,表明钙能降低铅的毒性效应.

This study was to investigate the toxic effects of interaction between calcium( Ca) and lead( Pb) on crucian. It was divided into five groups,i. e.,control group( Group Ⅰ),Pb2+alone exposed group( 0. 1 mg·L-1,Group Ⅱ),and Pb2+combined with Ca2+groups( 0. 1mg·L-1Pb2++2 mmol·L-1Ca2+( Group Ⅲ),0.1 mg·L-1Pb2++ 4 mmol·L-1Ca2+( Group Ⅳ),0.1 mg·L-1Pb2++ 6 mmol·L-1Ca2+( Group Ⅴ)). After exposed for 35 days,concentrations of Pb2+in the muscle and gill and the total anti-Oxidative capacity( T-AOC) as well as levels of catalase( CAT) and malondialdehyde( MDA) in the hepatopancreas were assessed. The results showed that Pb2+levels in the muscle and gill of Group II and Group III increased( p<0. 05) and its level in the muscle of Group V decreased( p<0. 05) compared to Group I. Compared with Group II,Pb2+levels in the muscle and gill of Group IV and Group V decreased significantly( p <0. 05). As far as the CAT activity was concerned,its activity in Group II was lower than that in Group I( p <0. 05),while its level in other three groups( Group III,Group IV and Group V) was higher than that in Group I( p<0. 05),respectively. Compared with Group II,the CAT activity in Group IV and Group V increased significantly( p<0. 05). Regarding the T-AOC,its level in three groups( II,III and V) was significantly lower( p<0. 05) than that in Group I,but T-AOC level in three groups( III,IV and V) was significantly higher( p<0. 05) than that in Group II,respectively. As for MDA,its content in other four groups( II,III,IV and V) was higher( p<0. 05) than that in Group I,respectively,while its level in three groups( III,IV and V) was lower( p <0. 05) than that in Group II,respectively. In summary,Ca2+can lower the accumulation of Pb2+in the muscle and gill of crucian and increase the anti-Oxidative ability in the hepatopancreas by the interaction between Ca2+and Pb2+,which demonstrated that Ca2+can alleviate the toxic effect of Pb2+.