HPLC法测定归龙筋骨宁胶囊中士的宁含量

Determination of Strychnine in Guilongjinguning capsules by HPLC

目的:建立归龙筋骨宁胶囊中士的宁的HPLC含量测定方法。方法:采用Thermo HTOERSIL Silica色谱柱(250mm×4.6mm,5μm),流动相为正己烷-二氟甲烷-甲醇-三乙胺(42.5:42.5:5.5:0.1),流速1.0ml/min,检测波长为254nm。结果:线性范围0.0660μg～0.6600μg(r=0.9999),呈良好线性关系,士的宁的平均加样回收率为99.38%,RSD=0.74%。结论:本方法操作简便,分离效果好,结果准确。

Objective:To establish a high performance liquid chromatography(HPLC)method for determination of strychnine in guilongjinguning capsulos.Methods:Silica gel was used as the stationary phase,n-hexane- dichloromethane-methanol-triethylamine(42.5:42.5:5.5:0.1)as the mobile phase,with the flow rate of 1.0ml per minute and detection wavelength at 254nm.Results:The linear range was from 0.0660μg to 0.6600μg(r=0.9999)and the linear correlation was well.The mean recovery of strychnine in guilongjinguning capsulos was 99.38%and RSD was 0.74%.Conclusion:This method was convenient and accurate.The abstraction effect was fine.