白蛋白过负荷对足细胞骨架蛋白α-actinin-4的影响

The Effection of Overloaded Albumin on α-actinin-4 of Podocytes

目的:观察足细胞损伤及药物干预后细胞骨架蛋白mRNA的变化,以进一步研究足细胞病的发病机制。方法:体外培养条件永生化小鼠足细胞,设立分组:对照组、足细胞损伤组(BSA 20mg/ml)、地塞米松组(地塞米松0.1umol/L+白蛋白20mg/ml),分别于12h、24h、48h检测足细胞内α-actinin4 mRNA表达变化。结果:足细胞损伤组足细胞内α-actinin4 mRNA水平随时间变化逐渐降低,地塞米松组先降低后升高。结论:地塞米松可以改善足细胞损伤后骨架蛋白α-actinin-4的mRNA表达的变化。

Objective:To observe the change of the mRNA of cytoskeletal protein in the podocyte cell after injured and medicine, and to explore the possible mechanism of podocyte injury. Methods:Mouse podocytes were cultured in vitro.Podocytes were divided into three groups: control, BSA overload(20mg/ml) and dexamethasone (BSA20 mg/ml and dexamethasone 0.1umol/L). PT-PCR was used to detect the levels ofα-actinin-4 mRNA when 12h, 24h and 48h. Results:The expression level was decreasd steadily with the change of time in BSA overload . Otherwise in dexamethasone, the expression level ofα-actinin4 mRNA appeared to have a decline trend initialiy ,then apppear an ascent with the change over time. Conclusion:Dexamethasone could change the levels of mRNA after podocytes damaged.