乙肝肝炎病毒大蛋白检测的临床意义

Clinical Significance of Test Large Protein on Hepatitis B Virus

目的:通过检测乙型肝炎病毒表面大蛋白(HBV-LP),乙型肝炎病毒免疫标识物和DNA,探讨乙型肝炎病毒大蛋自(HBV-LP)检测对于判断乙型肝炎病毒(HBV)复制的意义。方法:采用荧光实时定量PCR检测164例乙型肝炎病人血清病毒DNA、ELISA检测乙肝病毒表面大蛋白和HBe Ag。结果:乙肝病毒大蛋白(HBV-LP)的检出率和HBV DNA的检出率无显著差异,并且和HBV DNA拷贝数的对数值有相关性。在HBV DNA阳性的患者中,HBV-LP的阳性率高于HBe Ag,有统计意义。结论:乙肝病毒表面大蛋白检测与HBV DNA检测有很好的一致性,能反映乙肝病人机体乙型肝炎病毒的复制情况,可以作为检测乙肝病毒复制的新的指标。

Objectives:To investigate the significance of hepatitis B large protein (HBV-LP) detection and the prediction of viral replicatin throuth detection HBV-LP,HBV DNA and HBV marker. Methods:HBV-LPand HBV marker level were measured by en-zyme linked immunosorbent assay (ELISA) in 164 cases of infected serum and serum HBV DNA level was quantitatively detected by real-time polyerase chain reaction(PCR).The pisitive rate of HBV-LP was more high than HBeAg in the patient with HBV DNA. Re-sults:No significent differences were found in the positive rate between LP and the levels of HBV DNA and there was positive correla-tion between them. Conclusions:HBV-LP has a good consistency with HBV DNA ,it can reflact the dulicate of HBV in patient and can be used as a new indicator of viral replication was helpful complementarity to HBV DNA.