提高平末端连接效率的研究

The Study to Improve the Efficiency of Ligation of Blunt-ended DNA

在平末端连接反应中 ,为了提高连接效率 ,研究载体与片段的分子量比、载体与片段的摩尔比、连接反应体积、连接反应的温度、连接反应时间长短等条件对连接效率的影响 ,发现这些因素都会直接影响连接效率。因此在做不同的平末端连接反应时 ,应该把上述因素协同起来考虑。此外 。

It is important to improve the efficiency of ligation reaction, especially for joining of blunt-ended DNA which is universal applicated, however, with lower efficiency. In our study, We draw a conclusion that the mol-ratio of vector and insert?the volume of ligation reaction?the temperature and time of ligation reaction etc. All can influence the efficiency of ligation, so we should think of these factors together.