新城疫3组基因亚单位疫苗的免疫原性试验

Immunogenicity of Three Subunit Vaccine of ND

将 3个融合表达重组菌 p GHN/ DE3、p GF/ DE3和 p GF/ E.coli复苏后进行酶切鉴定 ,确认无误后均经 1m mol/L 的 IPTG诱导 ,然后分组 3次免疫鸡。试验鸡血清经血球凝集抑制试验 (HI)和微量细胞中和试验 (SN)来检测 HI抗体和 SN抗体。结果表明 :重组菌 p GHN/ DE3所生产的亚单位苗免疫鸡后 ,可激发较高的 HI抗体 ,但激发的中和抗体较弱。与 系 NDV常规疫苗相比 ,3个重组大肠杆菌基因工程苗所诱发的中和抗体效价较弱。对各组中起关键作用的中和抗体进行方差分析表明 ,3个基因工程菌所诱发的中和抗体与常规疫苗免疫组和基础免疫对照相比 ,差异显著。因此 ,尽管 3种基因工程苗产生了中和抗体 ,但中和抗体效价上升缓慢 ,HI抗体效价上升较快。

Three recombinant bacteria(pGHN/DE3,pGF/DE3 & p GF/E.coli)were reanimated and identified to include the fragements of HN or F gene of NDV.Then all the bacteria were induced with 1 mmol/L IPTG,and the expr essing products of the transformed E.coli were inoculated into chickens intr amuscularly.Antibody detection with HI and SN indicated that the HI antibody lev els were as high as those elicited with commercially available vaccines exceptio n of the lower SN antibody level.