一种直接从生物膜中萃取细胞外高分子有机物的方法

NOVEL METHOD EXTRACTING EXTRACELLUAR POLYMERIC SUBSTANCES FROM INTACT BIOFILMS

引言 生物膜(biofilm)是由微生物、细胞外高分子有机物(EPS)和惰性物质构成的多孔介质.最近研究发现,EPS的组成和含量不仅影响生物膜的内部结构和微生物活性,而且对基质在膜内的传质机理也有较大影响[1,5].

It is very important to find a effective method to extract the extracelluar polymeric substances (EPS) including polysaccharides, proteins and DNA, which have a significant role in the metabolism of the cells and the mass transfer of substrate within the biofilms, from biofilms without destroying its structure. In this study, a novel method to extract the EPS from intact biofilm was set up, based on the enhancement of external alternating electric field. The results showed that the current had insignificant effect on the cells structure, but it could enhance the extraction rate when charged extractant is used. In the range from 10 Hz to 500 Hz, the extraction rate increased with current frequency.