土壤中固氮菌的分离纯化与性质鉴定

Isolation,purification and property identification of Azotobacter in the soil

采用阿斯贝培养基进行菌种分离纯化,再通过吲哚试验、糖发酵试验等9个试验对其进行生理生化鉴定,最终得到一株革兰氏阴性,无芽孢的短杆状固氮菌。试验为微生物固氮菌肥生产中菌种的筛选提供参考,为商业化生产优质的生物固氮菌肥提供基础。

In this experimentation,Ashby medium was used to seperate and purify the strains,and through a series of laboratory experiments, such as indol test, suger fermentation test and other seven tests the strains were identified. At last,a G-and non- sporiferous bacillus was got. This experimentation is good for screening out the Azotobacter from the bacteria manure,and it is also the basis of producing the high quality nitrogen-fixing bacteria manure.