摘要
本文以气相色谱法测定人体中苯代谢物——尿酚,样品以85%H_3PO_4水解,然后游离酚萃取入CS_2层,取萃取液进样色谱仪作定量分析,测定数据表明,长时间与苯接触的人引起尿酚含量增加。从水层到CS_2层的萃取率:苯酚为28.85±1.07%,甲酚为66.68±0.92%。尿酚含量可作为与苯接触的指标,本文为调查苯污染情况提供了一简便可靠的方法。
This paper describes a GC method developed for determining the content of benzene metabolite in human urine. 85% H_3 PO_4 solution was added to the Sample to hydrolyse the conjugated phenols, and the phenols freed were extracted by CS_2.The CS_2 extract was then subjected to GC assay of phenol and p-cresol. Experimental data show that long contact with benzene will cause a high phenol content in urine. The rate of extraction of phenols from aqueous layer to the CS_2 layer is found to be 28.85±1.07% and 66.68±0.92% for phenol and p-cresol respectively.This method is recommended to be used in the measurement of phenol and p-cresol in urine, the result of which can be taken as an index of benzene exposure.
