口服西地那非对老龄大鼠心脏及阴茎组织凋亡的影响

Oral Administration of Sidenafil on Tissue Apoptosis of Heart and Penis of Aged Rats

目的探讨口服西地那非对老龄大鼠心脏及阴茎组织凋亡的影响。方法 30只大鼠平均分为两组,实验组给予西地那非灌胃(12 mg/kg),对照组生理盐水灌胃(1.6 ml/kg),每周3次,共8周。取心脏及阴茎组织,检测诱导型一氧化氮合酶(induced nitricoxide synthase,iNOS)的表达及细胞凋亡率。结果实验组灌胃后抓腮、爬高、追逐动作明显增多。实验组、对照组心脏组织iNOS表达的平均吸光度(optical density,OD)值分别为(0.32±0.04)、(0.40±0.05)(P>0.05),细胞凋亡率分别为(3.65±1.20)%、(2.81±0.78)%(P>0.05);阴茎组织iNOS的OD值分别为(0.37±0.04)、(0.23±0.03)(P<0.05),细胞凋亡率分别为(3.95±0.72)%、(1.43±0.69)%(P<0.01)。结论老龄大鼠长期服用西地那非后性行为增多,心脏组织iNOS表达及细胞凋亡变化不大;阴茎组织iNOS表达增多,细胞凋亡现象明显增加。

Objective To investigate tissue apoptosis effect of oral administration of Sidenafil on heart and penis of the aged rats.Methods Thirty rats were divided into two groups randomly:experimental group with Sidenafil orally(12mg/kg)and control group with Saline(1.6 ml/kg)for three times a week.After 8 weeks,the heart and penis were harvested to test induced nitricoxide synthase(iNOS)expression by immunohistochemistry and cell apoptosis by TdT-mediated dUTP-X nick end labeling(TUNEL).Results After oral Sidenafil administration,the occurrence of some actions such as grasping cheeks,climbing,chasing increased in experimental group.For the experimental and the control groups,iNOS expression in heart tissue was(0.32±0.04),(0.40±0.05)(P>0.05),and cell apoptosis rate was(3.65±1.20)%,(2.81±0.78)%(P>0.05);in penis tissue iNOS expression was(0.37±0.04),(0.23±0.03)(P<0.05)and the cell apoptosis rate was(3.95±0.72)%,(1.43±0.69)%(P<0.01),respectively.Conclusion After oral administration of Sidenafil for some time,the occurrence of rats' sexual behaviors can be increased,and iNOS expression and cell apoptosis in their penis increase obviously which is not seen in their hearts.