人类微小RNA196a在早期胃癌中异常表达的生物学意义

Differential Expression of Hsa-mi R-196a in Early Gastric Cancer and Its Biological Significance

目的明确人类微小RNA196a(hsa-mi R-196a)在早期胃癌中异常表达模式,探讨其异常表达在早期胃癌发生与发展过程中的生物学作用。方法收集作者医院57例临床标本的包括52例早期胃癌患者手术及全血标本和5例非癌患者全血标本,以混合的正常人全血标本为对照,采用实时定量聚合酶链反应(polymerase chain reaction,PCR)检测hsa-mi R-196a在早期胃癌患者中的表达水平。利用生物信息学数据库预测hsa-mi R-196a调控的靶基因及信号通路。结果在52例早期胃癌标本中,44例(44/52,84.6%)手术标本高表达hsa-mi R-196a;42例(42/52,80.8%)全血标本高表达hsa-mi R-196a。利用生物信息学方法获得了211个可能受hsa-mi R-196a所调控的靶基因,这些靶基因参与了多条肿瘤相关信号通路。结论人类微小RNA196a在早期胃癌中高表达,这一表达模式可能通调控多条肿瘤相关信号通路参与胃癌的早期进展。

Objective To detect the differential expression pattern of hsa-mi R-196 a in early gastric cancer(GC),and discuss the molecular machenisms of its role in the process of early GC.Methods A total of 57 cases were selected,including52 surgical samples and matched blood samples from early GC patients,and 5 blood samples as control from non-cancer patients.Real-time polymerase chain reaction(PCR) was used to detecte the relative expression level of hsa-mi R-196 a.Meanwhile,bioinformatics databases were used to predict the target genes and correspondent signaling pathways regulated by hsa-mi R-196 a.Results Hsa-mi R-196 a was up-regulated in 44 of 52(84.6%) surgical samples,and 42 of 52(80.8%) blood samples in early GC.A total of 211 target genes were predicted for further study.These genes regulated by hsa-mi R-196 a participated in some of the cancer related pathways.Conclusion Hsa-mi R-196 a is up-regulated in early GC.This overexpressed pattern of hsa-mi R-196 a may play a role in the process of early GC by regulating multipy cancer related signaling pathways.