益气活血方对佐剂性关节炎大鼠滑膜及血清中白细胞介素的影响

Influence of the Therapy of Tonifying QI and Eliminating Blood Stasis Interleukin in Adjuvant Arthritis Rats

目的观察益气活血方对佐剂性关节炎(adjuvant arthritis,AA)大鼠血清与滑膜中白细胞介素15(interleukin15,IL-15)、IL-27、IL-33的影响。方法建立AA大鼠模型,设立正常对照组、模型组、益气活血组和甲氨喋呤组进行对比观察,用药3周过程中检测各组大鼠关节炎指数,于用药3周后观察血清及滑膜液中IL-15、IL-27、IL-33的表达。结果益气活血方组能显著降低关节炎指数,并下调大鼠血清及滑膜液中IL-15、IL-33的表达,上调大鼠血清及滑膜液中IL-27的表达,与甲氨喋呤组效果相当。结论 IL-15、IL-27、IL-33与类风湿关节炎发病具有一定的相关性,益气活血方通过下调IL-15、IL-33的表达,上调IL-27的表达,起到治疗类风湿性关节炎(rheumatoid arthritis,RA)的作用。

Objective To observe the effect of the therapy of tonifying QI and eliminating blood on interleukin15(IL-15),IL-27,IL-33 in adjuvant arthritis(AA)rats.Methods AA rat model was established.The rats were randomly divided into six goups:normal,model,tonifying qi and eliminating blood and methotrexate(MTX)treatment.The influences of therapy to the arthritis index,IL-15,IL-27,IL-33 in serum and synovial fluid were observed.Results The therapy of tonifying qi and eliminating blood distinctly attenuated the arthritis index,decreased IL-15,IL-33 and increased IL-27 in serum and synovial fluid,the effects of which were similar to MTX.Conclusion The results demonstrate that IL-15,IL-27 and IL-33 are associated with rheumatoid arthritis.The mechanism of the therapy of tonifying qi and eliminating blood stasis in treating AA maybe increase IL-27 and decrease IL-15,IL-33 of AA rats,which reflects the advantages of Chinese medicine prescription under the direction of the treatment principles and methods of TCM.