南方红豆杉枝叶与果实中6种紫杉烷类化合物含量分析

Content analysis on 6 kinds of taxanes in branches, leaves and fruits of Taxus chinensis var.mairei

以南方红豆杉的枝、叶、果实为材料,建立了高效液相色谱法同时测定6种紫杉烷类化合物(紫杉醇、三尖杉宁碱、7–表–10–去乙酰紫杉醇、7–表–紫杉醇、巴卡亭III、10–去乙酰巴卡亭III)含量的方法。结果表明:以甲醇–乙腈–水(体积比为25∶36∶39)为流动相,等度洗脱,检测波长227 nm,6种紫杉烷类化合物在35 min内完全分离;南方红豆杉的枝、叶、果实中6种紫杉烷类化合物含量存在差异,其中,紫杉醇和7–表–10–去乙酰紫杉醇在种胚中含量最高,分别为866.47、722.50μg/g,10–去乙酰巴卡亭III、巴卡亭III和7–表–紫杉醇在叶中的含量最高,分别为301.20、234.08、11.74μg/g,三尖杉宁碱含量最高的部位是枝,为392.69μg/g。

The branches, leaves and fruits of Taxus chinensis var.mairei from Dawei mountain in Liuyang city were used as material, to establish a high performance liquid chromatograph(HPLC) method for simultaneous determination of 6 taxanes, namely, paclitaxel, cephalomannine(CE), 7– epi–10–deacetyl paclitaxel( 7–epi–10–DAT), 7–epi– paclitaxel, baccatin III and 10–deacetyl baccatin III(10–DAB). The result indicated that six kinds of taxanes were thoroughly separated within 35 min under the condition of using methanol-acetonitrile-water(volume rate of 25∶36∶39) as mobile phase through isocratic elution and the detection wavelength of 227 nm. The contents of six taxanes in the branches, leaves and fruits of Taxus chinensis var.mairei were differences, which the paclitaxel and 7– epi–10–deacetyl paclitaxel were the highest(866.47 、722.50 μg/g) in embryo, the 10–deacetyl baccatin III, baccatin III and 7–epi– paclitaxel were the highest(301.20、234.08、11.74 μg/g) in leaves, while the highest contents of cephalomannine was in branches(392.69 μg/g).