湖南省柿树叶片中黄酮类化合物含量的分析

Analysis of Flavonoids Content in Diospyros kaki Leaves in Hunan

通过建立稳定的柿树叶片黄酮类化合物HPLC分析方法,初步探讨柿树叶片黄酮类化合物的代谢累积规律,确定适宜的柿树叶片采收时期;同时检测分析了湖南省不同柿树品种的柿树叶片黄酮类化合物含量。结果表明,试验建立的HPLC分析方法稳定、可靠、重现性好,柿树叶片黄酮类化合物各物质峰之间分离度好;根据试验得出的柿树叶片黄酮类化合物代谢累积规律,确定适宜的柿树叶片采收时期为3月份的新叶萌发期和9~10月的果实成熟期;湖南省内7个地区17个不同柿树品种之间柿树叶片黄酮类化合物含量都有差异,其中含量最高的品种为次郎甜柿,含量最低的为野生腰带柿。

Thisstudy w asto detectflavonoidsin D iospyroskakileavesby H PLC and discusstheiraccum ulation therein, in orderto find the appropriate tim e for harvesting D iospyros kakileaves and determ ine leaf flavonoids contentin differentD iospyros kakivarieties in H unan. The results are as follow s: The H PLC analysis m ethod is stable, reliable and of good repeatability; the appropriate tim e for harvesting D iospyros kakileaves is in M arch and Septem berand O ctoberin accordance w ith the leafflavonoids contentm etabolism and accum ulation;and there are som e differences in leaf flavonoids contentam ong 17 D iospyros kaki varieties in 7 prefectures of the province, the highest contentin C iLang Persim m on and the low estin Y ao D aiShiC rude persim m on.