雪莲果试管苗快繁技术研究

Rapid Propagation of Yacon Plantlets in vitro

以雪莲果(SmallanthusSonchifolius(Poepp.End1.)H.Robinson)带腋芽的茎段为试验材料,研究雪莲果试管苗快繁技术。结果表明:MS+2,4-D 0.15mg·L-1+蔗糖(30g·L-1)+琼脂(6g·L-1)对雪莲果的茎段成苗效果最佳,成苗率达到95%,20d生根率100%,平均根长6.4cm,培养25d,平均苗高6.2cm;以腐质土:珍珠岩=3:1为基质,对组培苗进行移栽,成活率可达97%。

In this study,yacon stem s with axillary buds were used as experim entalm aterials to explore the rapid propagation of yacon in suitable conditions.The resultsshowthatM S + 2,4-d 0.15m g/L + sucrose (30g/L)+ agar(6g/L)wasofthe optim aleffecton plantletssprouted from the yacon stem s with the rooting rate at100% and average rootlength of 6.4cm after 20 d,and the seedling rate at95% and average heightof6.2 cm after25 d;and the garden soil∶coarse sand∶peat= 1∶1∶1 as a substrate was used fortissue -cultured plantlets to be transplanted on,the seedling survivalrate was97% .