杨梅酒对小鼠腹泻的防治作用及其作用机理

The Anti-diarrheal Effect and Mechanism of Chinese Bayberry Wine on Diarrhea of Mice

为研究杨梅酒对腹泻的防治效果及其作用机制,采用生长曲线法研究了杨梅酒对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌生长增殖的影响,分析乙酸乙酯、乙醚等溶剂萃取的杨梅酒活性组分的体外抑菌效果,并以腹泻模型小鼠为对象,探究了不同杨梅酒萃取物对小鼠腹泻的防治效果。结果表明,与生理盐水和高粱烧相比,杨梅酒对致病菌生长增殖的抑制作用十分明显且杨梅酒乙酸乙酯提取物的抑菌作用最强。杨梅酒乙酸乙酯提取物对模型小鼠腹泻的防治效果最佳,能够显著抑制NF-κB信号通路的激活,抑制IL-8、TNF-α等炎症因子的表达,说明杨梅酒中活性成分可通过抑制NF-κB信号通路,发挥抗炎作用,这为阐释杨梅酒防治腹泻的分子机制提供了理论参考,为进一步探明杨梅酒中具有抗菌消炎作用的活性成分及其药理作用提供了新线索。

To study the control effects of Chinese bayberry wine on the diarrhea and investigate its mechanism,growth curve method was used to determine the effects on E. coli,S. aureus,and B. subtilis. The antibacterial activities of functional ingredients extracted by ethyl acetate,ether or water were analyzed by in vitro experiments. The anti-diarrheal function of the active ingredients was investigated in a mice model. The results showed Chinese bayberry wine significantly inhibited the growth and proliferation of the pathogenic bacteria compared to normal saline or kaoliang spirit,the ethyl acetate extract showed stronger antibacterial and anti-diarrheal action,which could obviously suppress the activation of NF-κB signaling pathway and downregulate the expression of IL-8 and TNF-α. In conclusion,Chinese bayberry wine played important roles on anti-inflammation by inhibiting the activation of NF-κB signaling pathway. The results of this study will provide important references for disclosing the anti-diarrheal molecular mechanism of Chinese bayberry wine,and supply new clues for exploring the anti-inflammatory active ingredient and its pharmacological action.