NaOH协同辐照预处理提高稻草纤维素酶解转化率

The Enzymatic Hydrolysis Conversion Rate of Cellulose in Rice Straw Improved by Using NaOH Solution Collaborated With Irradiation

为降低预处理成本,提升稻草纤维素的酶解效果,采用NaOH协同^(60)Co-γ射线辐照处理稻草,研究其对稻草中纤维素酶解转化率的影响,利用扫描电镜(SEM)、红外光谱(FT-IR)分析协同预处理对稻草微观结构的影响,并通过正交试验对协同预处理条件参数进行优化。结果表明,NaOH协同辐照预处理能显著提高稻草纤维素酶解转化率,优化后的预处理条件为400 kGy辐照剂量的稻草结合2%NaOH溶液,固液比1∶15,50℃条件下处理4 h,按照10 FPU·g^(-1)加入纤维素酶溶液,于50℃、130 r·min^(-1)条件下气浴恒温振荡,酶解24 h后,其稻草纤维素转化率达到78.54%±1.20%。本研究结果为农业废弃物资源能源化高效利用提供了技术支撑和理论依据。

To reduced the pretreatment costs,and improved the enzymolysis effect of cellulose in rice straw,the rice straw pretreated by using NaOH solution collaborated with irradiation by 60Coγ-rays were used for studying the effect of enzymatic hydrolysis conversion rate of cellulose in rice straw.Scanning electron microscope(SEM)and infrared spectroscopy(FT-IR)were used for scaning the microstructure of the rice straw that pretreated by above method.The results showed that the enzymatic hydrolysis conversion rate of cellulose in rice straw were significantly improved by the pretreatment.Orthogonal test showed that the rice straw under the pretreatment conditions of 400 kGy irradiation dose and 2%NaOH solution in 50℃for 4 h with the solid-to-liquid ratio of 1∶15,and the enzymatic hydrolysis conversion rate of cellulose in treated rice straw oscillated under Gas bath constant temperature with 130 r·min-1 in 50℃for 24 h after added cellulase with 10 FPU·g-1 was 78.54%±1.20%,This result provided the theoretical basis and technical support for utilizing the agricultural waste biomass resources.