^(60)Co-γ射线辐射对2种露地菊叶片生理生化特性的影响

Effect of ^(60)Co-γ Ray on Physiological and Biochemical Characteristics of Two Kinds of Chrysanthemum grandi florum leaves

为探究露地菊对^(60)Co-γ射线辐射的耐受性,以露地菊品种东林瑞雪和粉翎的生根脚芽为试验材料,研究不同辐照处理对2种露地菊品种叶片生理生化指标的影响。结果表明,随着辐照剂量的增加,东林瑞雪和粉翎叶绿素含量和可溶性蛋白含量均呈下降趋势;脯氨酸和丙二醛(MDA)含量均呈逐渐增加的趋势;超氧化物歧化酶(SOD)活性均呈先增加后降低的趋势;过氧化物酶(POD)均呈逐渐增加的趋势;东林瑞雪过氧化氢酶(CAT)活性呈先增加后降低的趋势,粉翎的CAT活性呈逐渐增加的趋势。综上可知,粉翎对辐射的耐受能力强于东林瑞雪。本研究结果为露地菊辐射诱变研究提供了一定的理论依据和技术参考。

In order to investigate the tolerance of Chrysanthemum morifolium Ramat.to 60Co-ray radiation,rooting buds of Donglin Ruixue and Pink Ling(which were chrysanthemum varieties)were taken as experimental materials to study effects of different irradiation treatments on physiological and biochemical indexes of leaves of two kinds of dewdrop chrysanthemum varieties.The results showed that with the increase of radiation dose,the contents of chlorophyll content and the content of soluble protein of the two plants both showed a decreasing trend;the content of proline and malondialdehyde(MDA)increased gradually;the activity of superoxide dismutase(SOD)increased first and then decreased,while the activity of peroxidase(POD)increased continuously.The activity of catalase(CAT)in Donglin Reixue showed a trend of first increasing and then decreasing,while in Pink Ling showed an upward trend.The changes of the physiological and biochemical indexes of the two plants showed that‘Pink Ling‘is more resistant to radiation than‘Donglin Ruixue’.This study provides a theoretical basis and technical reference for the research of floral radiation mutagenesis.