基于不同倍性谷子主茎形态建成的相关内源激素调控差异分析

Differential Regulation of Endogenous Hormone on Stem Morphogenesis in Millet With Different Ploidy

为揭示谷子相关内源激素对主茎形态发生及株型构建的影响,以晋谷21号(DJG21)及其同源四倍体诱变株(TJG21)为试验材料,采用高效液相色谱-质谱(HPLC-MS)联用技术,研究拔节期不同倍性谷子主茎内源激素IAA、GAs、ABA、SA、JA的动态差异及其对主茎形态建成的影响。结果表明,DJG21和TJG21成株主茎节间数相同,均为15。DJG21和TJG21分别在播后67和75 d拔节完成。拔节前期TJG21主茎增长较DJG21快,后期二者无显著差异。DJG21染色体经诱变加倍后,TJG21主茎的ABA、JA、SA含量明显增加。TJG21主茎拔节与节间增长滞后与其ABA含量增高及(IAA+GAs)/ABA值降低密切相关。TJG21主茎SA及JA含量整体大于DJG21,前期JA含量增加更为明显,后期SA含量增加更为明显。DJG21和TJG21拔节期主茎内源激素含量及调控的差异造成不同倍性谷子生长发育进程的不同,是由遗传及环境因素双重影响下的形态发生过程。本研究结果不仅为人为构建激素平衡以调控谷子生长发育提供了一定的理论依据,也为不同倍性谷子拔节期转录组分析及相关基因发掘奠定了基础。

To reveal the influences of endogenous hormones on main stem morphogenesis and plant architecture formation millet,Jingu 21(DJG21)and its autotetraploid mutagenesis strain(TJG21)as the experimental materials,and high performance liquid chromatography-mass spectrometry(HPLC-MS)was carrired out to detect the dynamic differences of endogenous hormones in stem,including IAA,GAs,ABA,SA,JA,and its influence on main stem morphogenesis between two millet strains with different ploidy in elongation stage.The results showed that DJG21 and TJG21 have equal internodes,both were 15.It took 67 d for DJG21 stem to finish jointing,while TJG21 was 75 d.The main stem of TJG21 growed faster than that of DJG21 in early elongation stage.But there was no significant difference between them in later stage.The contents of ABA,JA and SA in the main stem of TJG21 increased obviously after chromosomes of DJG21 was mutated and doubled.The elongation hysteresis of TJG21 main stem and slow growth of its internodes were closely related to the increase of ABA content and the decrease of(IAA+GAs)/ABA value.As a whole,the contents of SA and JA in the main stem of TJG21 were higher than those in DJG21,and the increase of JA was more obvious in the early stage,while SA was more obvious in the later stage.The differences of endogenous hormone content and regulation between DJG21 and TJG21 during elongating stage leaded to different growth and development processes of millet with different ploidy,which is aprocess of morphogenesis involved with both genetic and environment factors.The results of this study not only provide a theoretical basis for the artificial construction of hormone balance to regulate the growth and development of millet,but also lay a foundation for transcriptional analysis of millet with different ploidy at jointing stage and identification of candidate genes.